Western Blot (WB)
(TBC1D10A Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TBC1D10A antibody detected the TBC1D10A protein (arrow).)
Rabbit anti-Human, Mouse TBC1D10A Polyclonal Antibody | anti-TBC1D10A antibody
TBC1D10A, ID (TBC1D10A, EPI64, TBC1D10, TBC1 domain family member 10A, EBP50-PDX interactor of 64kD, Rab27A-GAP-alpha) (Azide free) (HRP)
Gene Names
TBC1D10A; EPI64; TBC1D10; dJ130H16.1; dJ130H16.2
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TBC1D10A; Polyclonal Antibody; ID (TBC1D10A; EPI64; TBC1D10; TBC1 domain family member 10A; EBP50-PDX interactor of 64kD; Rab27A-GAP-alpha) (Azide free) (HRP); Rab27A-GAP-alpha; anti-TBC1D10A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-TBC1D10A antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
TBC1D10A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-267 amino acids from the Central region of human TBC1D10A.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(TBC1D10A Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TBC1D10A antibody detected the TBC1D10A protein (arrow).)
Western Blot (WB)
(TBC1D10A Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TBC1D10A antibody detected the TBC1D10A protein (arrow).)
Western Blot (WB)
(TBC1D10A Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the TBC1D10A antibody detected the TBC1D10A protein (arrow).)
Western Blot (WB)
(TBC1D10A Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the TBC1D10A antibody detected the TBC1D10A protein (arrow).)
Related Product Information for anti-TBC1D10A antibody
TBC1D10A acts as GTPase-activating protein for RAB27A, but not for RAB2A, RAB3A, nor RAB4A.
Product Categories/Family for anti-TBC1D10A antibody
References
Gao, Y., et al. J. Biol. Chem. 285(23):17938-17953(2010). Ishibashi, K., et al. Genes Cells 14(1):41-52(2009). Patino-Lopez, G., et al. J. Biol. Chem. 283(26):18323-18330(2008). Hanono, A., et al. J. Cell Biol. 175(5):803-813(2006). Itoh, T., et al. J. Biol. Chem. 281(42):31823-31831(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,935 Da
NCBI Official Full Name
TBC1 domain family member 10A isoform 2
NCBI Official Synonym Full Names
TBC1 domain family, member 10A
NCBI Official Symbol
TBC1D10A
NCBI Official Synonym Symbols
EPI64; TBC1D10; dJ130H16.1; dJ130H16.2
NCBI Protein Information
TBC1 domain family member 10A; rab27A-GAP-alpha; EBP50-PDZ interactor of 64 kD; EBP50-PDX interactor of 64 kDa
UniProt Protein Name
TBC1 domain family member 10A
Protein Family
UniProt Gene Name
TBC1D10A
UniProt Synonym Gene Names
EPI64; TBC1D10; EPI64 protein
UniProt Entry Name
TB10A_HUMAN
Uniprot Description
TBC1D10A: a GTPase-activating protein (GAP) for RAB27A, but not for RAB2A, RAB3A, nor RAB4A. Binds to the first PDZ domain of SLC9A3R1 and SLC9A3R2. Localizes to the microvilli-rich region of the syncytiotrophoblast. In melanocytes, located at the periphery of cells.
Protein type: GAPs, Rab; GAPs
Chromosomal Location of Human Ortholog: 22q12.2
Cellular Component: microvillus; plasma membrane
Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; PDZ domain binding
Biological Process: positive regulation of proteolysis; retrograde transport, endosome to Golgi
Research Articles on TBC1D10A
Similar Products
Product Notes
The TBC1D10A tbc1d10a (Catalog #AAA6353835) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TBC1D10A, ID (TBC1D10A, EPI64, TBC1D10, TBC1 domain family member 10A, EBP50-PDX interactor of 64kD, Rab27A-GAP-alpha) (Azide free) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TBC1D10A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TBC1D10A tbc1d10a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBC1D10A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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