Western Blot (WB)
(SPT13 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SPT13 antibody detected the SPT13 protein (arrow).)
Rabbit anti-Human SPT13 Polyclonal Antibody | anti-SPT13 antibody
SPT13, ID (SPATA13, Spermatogenesis-associated protein 13, APC-stimulated guanine nucleotide exchange factor 2) (AP)
Gene Names
SPATA13; ASEF2; ARHGEF29
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SPT13; Polyclonal Antibody; ID (SPATA13; Spermatogenesis-associated protein 13; APC-stimulated guanine nucleotide exchange factor 2) (AP); SPATA13; APC-stimulated guanine nucleotide exchange factor 2; anti-SPT13 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-SPT13 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SPT13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human SPT13.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(SPT13 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SPT13 antibody detected the SPT13 protein (arrow).)
Western Blot (WB)
(SPT13 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SPT13 antibody detected the SPT13 protein (arrow).)
Immunohistochemistry (IHC)
(SPT13 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPT13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SPT13 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPT13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(SPT13 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SPT13 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-SPT13 antibody
References
Ding, H., et al. Stroke 41(1):177-180(2010). Bristow, J.M., et al. J. Cell. Sci. 122 (PT 24), 4535-4546 (2009). Sagara, M., et al. Oncogene 28(10):1357-1365(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
141,436 Da
NCBI Official Full Name
spermatogenesis-associated protein 13 isoform 2
NCBI Official Synonym Full Names
spermatogenesis associated 13
NCBI Official Symbol
SPATA13
NCBI Official Synonym Symbols
ASEF2; ARHGEF29
NCBI Protein Information
spermatogenesis-associated protein 13
UniProt Protein Name
Spermatogenesis-associated protein 13
UniProt Gene Name
SPATA13
UniProt Synonym Gene Names
Asef2
UniProt Entry Name
SPT13_HUMAN
Research Articles on SPT13
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Product Notes
The SPT13 spata13 (Catalog #AAA6350916) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPT13, ID (SPATA13, Spermatogenesis-associated protein 13, APC-stimulated guanine nucleotide exchange factor 2) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPT13 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SPT13 spata13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPT13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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