Rabbit anti-Human SP1 Polyclonal Antibody | anti-SP1 antibody

SP1, CT (P692) (SP1, TSFP1, Transcription factor Sp1) (Azide free) (HRP)

Cat. Num. AAA6350133

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SP1; Polyclonal Antibody; CT (P692) (SP1; TSFP1; Transcription factor Sp1) (Azide free) (HRP); Transcription factor Sp1; anti-SP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SP1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 684-715 amino acids from the C-terminal region of human SP1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SP1 Antibody (C-term P692) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SP1 antibody detected the SP1 protein (arrow).)

Immunohistochemistry (IHC)

(SP1 Antibody (C-term P692) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP1 Antibody (C-term P692) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SP1 Antibody (C-term P692) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of SP1 Antibody (C-term P692) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-SP1 antibody

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Product Categories/Family for anti-SP1 antibody

Antibodies; Transcription Factors

References

Pan, Q., et al. Biochem. Biophys. Res. Commun. 401(2):306-312(2010). Mucha, M., et al. J. Neurosci. 30(40):13235-13245(2010). Imanishi, M., et al. Biochem. Biophys. Res. Commun. 400(4):625-630(2010). Jutooru, I., et al. J. Biol. Chem. 285(33):25332-25344(2010). Logotheti, S., et al. FEBS J. 277(14):3014-3027(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 38372901
• NCBI GeneID: 6667
• NCBI Accession #: NP_612482.2
• NCBI GenBank Nucleotide #: NM_138473.2
• UniProt Accession #: P08047; Q86TN8; Q9H3Q5; Q9NR51; Q9NY21; Q9NYE7; E4Z9M7; G5E9M8
• Molecular Weight: 75,824 Da
• NCBI Official Full Name: transcription factor Sp1 isoform a
• NCBI Official Synonym Full Names: Sp1 transcription factor
• NCBI Official Symbol: SP1
• NCBI Protein Information: transcription factor Sp1; specificity protein 1
• UniProt Protein Name: Transcription factor Sp1
• Protein Family: Sp110 nuclear body protein
• UniProt Gene Name: SP1
• UniProt Synonym Gene Names: TSFP1
• UniProt Entry Name: SP1_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Uniprot Description

SP1: a transcription factor of the Sp1 C2H2-type zinc-finger protein family. Phosphorylated and activated by MAPK. Dephosphorylation by PTEN inhibits DNA binding. Binds to p38 in the nucleus. Interacts with Huntingtin and TAFII130. Transcriptional activity of SP1 and TAFII130 disrupted in early Huntingtin's Disease.

Protein type: DNA-binding; Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein C-terminus binding; histone acetyltransferase binding; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; metal ion binding; histone deacetylase binding; double-stranded DNA binding; bHLH transcription factor binding; transcription factor binding; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; ossification; embryonic placenta development; transcription, DNA-dependent; viral reproduction; megakaryocyte differentiation; embryonic skeletal development; positive regulation of transcription, DNA-dependent; embryonic process involved in female pregnancy; rhythmic process; cellular lipid metabolic process; enucleate erythrocyte differentiation; liver development; trophectodermal cell differentiation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; lung development