Rabbit Sox10 Polyclonal Antibody | anti-Sox10 antibody

Sox10, ID (High Mobility Group HMG Transcription Factor) (Azide free) (HRP)

Cat. Num. AAA6349918

• Gene Names: SOX10; DOM; WS4; PCWH; WS2E; WS4C
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Sox10; Polyclonal Antibody; ID (High Mobility Group HMG Transcription Factor) (Azide free) (HRP); anti-Sox10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SOX10. Species Crossreactivity: mouse, rat
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Labeled with HRP.

• Applicable Applications for anti-Sox10 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: Requires HIER using sodium citrate buffer pH6.0 prior to staining.; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 231-259 of human SOX10, UniProt Accession #P56693. Species sequence homology: procine
• Conjugate: HRP
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse heart tissue lysate (15ug) using antibodyMBS6001934.)

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin-fixed, paraffin-embedded human brain tissue usingMBS6001934. HRP-conjugated secondary antibody and DAB staining were used.)

Immunohistochemistry (IHC)

(Immunohistochemistry staining of SOX10 in paraformaldehyde-fixed, paraffin-embedded human colon tissue sections using MBS6001934 (1:25). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with MBS6001934 (1:25) for 1 hour at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Product Categories/Family for anti-Sox10 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 5902104
• NCBI GeneID: 6663
• NCBI Accession #: NP_008872.1
• NCBI GenBank Nucleotide #: NM_006941.3
• UniProt Accession #: P56693; Q6FHW7; B4DV62
• Molecular Weight: 49911 MW
• NCBI Official Full Name: transcription factor SOX-10
• NCBI Official Synonym Full Names: SRY-box 10
• NCBI Official Symbol: SOX10
• NCBI Official Synonym Symbols: DOM; WS4; PCWH; WS2E; WS4C
• NCBI Protein Information: transcription factor SOX-10
• UniProt Protein Name: Transcription factor SOX-10
• Protein Family: Transcription factor
• UniProt Gene Name: SOX10

NCBI Description

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008]

Uniprot Description

SOX10: Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E). WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C); also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH); also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.

Protein type: Cell development/differentiation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; transcription coactivator activity

Biological Process: anatomical structure morphogenesis; regulation of transcription from RNA polymerase II promoter

Disease: Peripheral Demyelinating Neuropathy, Central Dysmyelination, Waardenburg Syndrome, And Hirschsprung Disease; Waardenburg Syndrome, Type 2e; Waardenburg Syndrome, Type 4c