Vitronectin Active Protein | VTN active protein

Vitronectin (Vitronectin V10 Subunit, Vitronectin V65 Subunit, VN, VNT, VTN, Complement S Protein, Epibolin, S Protein, S-Protein, Serum Spreading Factor, Somatomedin B, Somatomedin-B, V75)

Cat. Num. AAA634977

• Gene Names: VTN; VN; V75; VNT
• Applications: ELISA
• Purity: Purified; 90% by SDS-PAGE. Two bands in SDS-PAGE at 65kD and 75kD
• Synonyms: Vitronectin; Vitronectin (Vitronectin V10 Subunit; Vitronectin V65 Subunit; VN; VNT; VTN; Complement S Protein; Epibolin; S Protein; S-Protein; Serum Spreading Factor; Somatomedin B; Somatomedin-B; V75); VTN active protein

• Host: Human plasma
• Purity/Purification: Purified; 90% by SDS-PAGE. Two bands in SDS-PAGE at 65kD and 75kD
• Form/Format: Supplied as a liquid in 50mM sodium phosphate, 100mM sodium chloride, pH 7.4. No preservatives added.

• Applicable Applications for VTN active protein: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA, Immunization, Cell Attachment Assays and Electrophoresis..
• Activity: Active in cell attachment assays at 1ug/ml. Binds to heparin. Active in cell attachment of MG63 cells.
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

SDS-Page

(SDS-PAGE of purified V2200-01)

Related Product Information for VTN active protein

Vitronectin (S-protein) is a multi-functional plasma and extracellular matrix protein with defined activity in cell adhesion, thrombosis, complement activation, fibrinolysis, inflammation, and platelet adhesion. Vitronectin binds to other macromolecules and participates in regulatory circuits to control these processes. Some target macromolecules that interact with vitronectin are heparin, PAI-1, proteases such as thrombin and urokinase-type plasminogen activator (uPA), serine protease inhibitor-protease complexes, the uPA receptor, and a sub-class of integrin receptors on the surface of cells. Vitronectin is also applied as a thin coating on tissue-culture surfaces, which is useful to promote cell attachment, spreading, proliferation, and differentiation of many normal and neoplastic cells, and to study cell migration.

Product Categories/Family for VTN active protein

Molecular Biology; MB-Vitronectin

NCBI and Uniprot Product Information

• NCBI GI #: 88853069
• NCBI GeneID: 7448
• NCBI Accession #: NP_000629.3
• NCBI GenBank Nucleotide #: NM_000638.3
• UniProt Accession #: P04004; P01141; Q9BSH7; B2R7G0
• Molecular Weight: 54,306 Da
• NCBI Official Full Name: vitronectin
• NCBI Official Synonym Full Names: vitronectin
• NCBI Official Symbol: VTN
• NCBI Official Synonym Symbols: VN; V75; VNT
• NCBI Protein Information: vitronectin; epibolin; S-protein; somatomedin B; complement S-protein; serum spreading factor; serum-spreading factor
• UniProt Protein Name: Vitronectin
• Protein Family: Vitronectin
• UniProt Gene Name: VTN
• UniProt Synonym Gene Names: VN
• UniProt Entry Name: VTNC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]

Uniprot Description

VTN: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q11

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: heparin binding; integrin binding; protein binding; extracellular matrix binding; scavenger receptor activity; polysaccharide binding

Biological Process: receptor-mediated endocytosis; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell-matrix adhesion; negative regulation of blood coagulation; positive regulation of smooth muscle cell migration; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of receptor-mediated endocytosis; positive regulation of peptidyl-tyrosine phosphorylation; regulation of complement activation; innate immune response; cell adhesion mediated by integrin; immune response; cell adhesion