Interleukin 1 Receptor Antagonist Active Protein | IL1RN active protein

Interleukin 1 Receptor Antagonist (IL-1Ra)

Cat. Num. AAA634948

• Gene Names: IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
• Purity: Highly Purified; ~98% by RP-HPLC and SDS-PAGE
• Synonyms: Interleukin 1 Receptor Antagonist; Interleukin 1 Receptor Antagonist (IL-1Ra); IL1RN active protein

• Host: E Coli
• Purity/Purification: Highly Purified; ~98% by RP-HPLC and SDS-PAGE
• Form/Format: Supplied as a sterile filtered, lyophilized powder. No additives. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to >0.1mg/ml.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pro-Ser-Gly.

• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent inhibition of IL-1 stimulation of D10S cells was found to be 0.5ng/ml.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280nm using the absorbency value of 0.89 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics); 2. Analysis by RP-HPLC, using a calibrated solution of IL-8 as a reference standard.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

Related Product Information for IL1RN active protein

Interleukin-1 Receptor Antagonist is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning ~400kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Recombinant Human IL-1RA produced in E. coli is a non-glycosylated, N-terminal methionyl form of the human naturally-occurring polypeptide chain containing 153 amino acids and having a molecular mass of 17kD.

Product Categories/Family for IL1RN active protein

Molecular Biology; MB-Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 186386
• NCBI GeneID: 3557
• UniProt Accession #: P18510; Q14628; Q53SC2; Q7RTZ4; Q96GD6; Q9UPC0; A8K4G1
• Molecular Weight: 17kD
• NCBI Official Full Name: interleukin 1 receptor antagonist
• NCBI Official Synonym Full Names: interleukin 1 receptor antagonist
• NCBI Official Symbol: IL1RN
• NCBI Official Synonym Symbols: DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
• NCBI Protein Information: interleukin-1 receptor antagonist protein; IL1 inhibitor; IL1RN (IL1F3); type II interleukin-1 receptor antagonist; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra)
• UniProt Protein Name: Interleukin-1 receptor antagonist protein
• Protein Family: Interleukin-1 receptor antagonist protein
• UniProt Gene Name: IL1RN
• UniProt Synonym Gene Names: IL1F3; IL1RA; IL-1RN; IL-1ra; IRAP
• UniProt Entry Name: IL1RA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL1RN: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. The intracellular form of IL1RN is predominantly expressed in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cytokine; Vesicle

Chromosomal Location of Human Ortholog: 2q14.2

Cellular Component: extracellular space; cytoplasm; plasma membrane; intracellular

Molecular Function: interleukin-1 Type I receptor antagonist activity; interleukin-1 receptor binding; interleukin-1 Type II receptor antagonist activity; interleukin-1, Type II receptor binding; cytokine activity; interleukin-1, Type I receptor binding; interleukin-1 receptor antagonist activity

Biological Process: response to drug; positive regulation of JNK activity; negative regulation of heterotypic cell-cell adhesion; negative regulation of glutamate secretion; response to glucocorticoid stimulus; carboxylic acid metabolic process; response to lipopolysaccharide; female pregnancy; sensory perception of pain; fever; chronic inflammatory response to antigenic stimulus; memory; response to starvation; insulin secretion; acute-phase response; negative regulation of cytokine and chemokine mediated signaling pathway; immune response; response to activity; lipid metabolic process; negative regulation of membrane potential; negative regulation of cell migration; negative regulation of apoptosis

Disease: Gastric Cancer, Hereditary Diffuse; Microvascular Complications Of Diabetes, Susceptibility To, 4; Osteomyelitis, Sterile Multifocal, With Periostitis And Pustulosis