Rabbit anti-Human, Mouse SLC25A17 Polyclonal Antibody | anti-SLC25A17 antibody

SLC25A17, NT (SLC25A17, PMP34, Peroxisomal membrane protein PMP34, 34kD peroxisomal membrane protein, Solute carrier family 25 member 17) (FITC)

Cat. Num. AAA6348223

• Gene Names: SLC25A17; PMP34
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SLC25A17; Polyclonal Antibody; NT (SLC25A17; PMP34; Peroxisomal membrane protein PMP34; 34kD peroxisomal membrane protein; Solute carrier family 25 member 17) (FITC); Solute carrier family 25 member 17; anti-SLC25A17 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SLC25A17 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SLC25A17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23~53 amino acids from the N-terminal region of human SLC25A17.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SLC25A17 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). SLC25A17 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of SLC25A17 Antibody (N-term) in Mouse NIH-3T3 cell line lysates (35ug/lane). SLC25A17 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(SLC25A17 Antibody (N-term) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SLC25A17 antibody

This protein encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Studies suggest that this protein may regulate cytokine production, and thus play a key role in the control of the inflammatory response.

Product Categories/Family for anti-SLC25A17 antibody

Antibodies; Transport Proteins

References

McGeachie, M., et.al., Circulation 120 (24), 2448-2454 (2009). Kelly, E., et.al., J. Biol. Chem. 284 (25), 16891-16897 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 47678685
• NCBI GeneID: 10478
• UniProt Accession #: O43808; Q5TFL0; Q9UGW8; Q9UGY7; A8KA59
• Molecular Weight: 34,567 Da
• NCBI Official Full Name: SLC25A17
• NCBI Official Synonym Full Names: solute carrier family 25 (mitochondrial carrier; peroxisomal membrane protein, 34kDa), member 17
• NCBI Official Symbol: SLC25A17
• NCBI Official Synonym Symbols: PMP34
• NCBI Protein Information: peroxisomal membrane protein PMP34; solute carrier family 25 member 17
• UniProt Protein Name: Peroxisomal membrane protein PMP34
• Protein Family: Peroxisomal membrane protein
• UniProt Gene Name: SLC25A17
• UniProt Synonym Gene Names: PMP34
• UniProt Entry Name: PM34_HUMAN

NCBI Description

This gene encodes a peroxisomal membrane protein that belongs to the family of mitochondrial solute carriers. It is expressed in the liver, and is likely involved in transport. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

Function: Peroxisomal transporter for multiple cofactors like coenzyme A (CoA), flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN) and nucleotide adenosine monophosphate (AMP), and to a lesser extend for nicotinamide adenine dinucleotide (NAD+), adenosine diphosphate (ADP) and adenosine 3',5'-diphosphate (PAP). May catalyze the transport of free CoA, FAD and NAD+ from the cytosol into the peroxisomal matrix by a counter-exchange mechanism. Inhibited by pyridoxal 5'-phosphate and bathophenanthroline in vitro. Ref.10 Ref.12

Subunit structure: Interacts (via N- and C-terminus peroxisomal targeting regions) with PEX19; the interaction occurs with the newly synthesized SLC25A17 in the cytosol. Ref.7 Ref.9 Ref.11

Subcellular location: Cytoplasm. Peroxisome membrane; Multi-pass membrane protein Ref.8 Ref.9 Ref.11.

Tissue specificity: Ubiquitous. Expressed in liver. Ref.12

Domain: The N- and C-terminal portions are exposed to the cytoplasm. Lacks a typical peroxisomal sorting signal. A region between helical transmembrane domains (TM) 4 and 5 and TM1-TM3 or TM4-TM6 are necessary for the peroxisome-targeting activity.

Sequence similarities: Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]Contains 3 Solcar repeats.

Caution: Was first identified as a peroxisomal ATP transporter (Ref.10). However, later experiments showed that it acts as a peroxisomal transporter for multiple cofactors (Ref.12).