Rabbit anti-Human SIT1 Polyclonal Antibody | anti-SIT1 antibody

SIT1, CT (SIT1, SIT, Signaling threshold-regulating transmembrane adapter 1, SHP2-interacting transmembrane adapter protein, Suppression-inducing transmembrane adapter 1, gp30/40) (PE)

Cat. Num. AAA6347856

• Gene Names: SIT1; SIT; SIT-R
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SIT1; Polyclonal Antibody; CT (SIT1; SIT; Signaling threshold-regulating transmembrane adapter 1; SHP2-interacting transmembrane adapter protein; Suppression-inducing transmembrane adapter 1; gp30/40) (PE); gp30/40; anti-SIT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SIT1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the C-terminal region of human SIT1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SIT1 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the SIT1 antibody detected the SIT1 protein (arrow).)

Related Product Information for anti-SIT1 antibody

Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.

Product Categories/Family for anti-SIT1 antibody

Antibodies; Transmembrane Proteins

References

Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009). Tedoldi, S., et al. Blood 107(1):213-221(2006). Tao, W.A., et al. Nat. Methods 2(8):591-598(2005). Tao, W.A., et al. Nat. Methods 2(8):591-598(2005). Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 7657577
• NCBI GeneID: 27240
• NCBI Accession #: NP_055265.1
• NCBI GenBank Nucleotide #: NM_014450.2
• UniProt Accession #: Q9Y3P8; B2RBP9
• Molecular Weight: 21,126 Da
• NCBI Official Full Name: signaling threshold-regulating transmembrane adapter 1
• NCBI Official Synonym Full Names: signaling threshold regulating transmembrane adaptor 1
• NCBI Official Symbol: SIT1
• NCBI Official Synonym Symbols: SIT; SIT-R
• NCBI Protein Information: signaling threshold-regulating transmembrane adapter 1; SHP-2 interacting transmembrane adaptor protein; SHP2 interacting transmembrane adaptor; SHP2-interacting transmembrane adapter protein; SHP2-interacting transmembrane adaptor protein; gp30/40; suppr
• UniProt Protein Name: Signaling threshold-regulating transmembrane adapter 1
• Protein Family: Signaling threshold-regulating transmembrane adapter
• UniProt Gene Name: SIT1
• UniProt Synonym Gene Names: SIT
• UniProt Entry Name: SIT1_HUMAN

Uniprot Description

SIT: a low molecular weight transmembrane adaptor protein. A disulfide-linked homodimer. Expressed in lymphocytes. Following phosphorylation by Src and possibly Tec kinases, recruits the SH2 domain-containing tyrosine phosphatase SHP2 via an immunoreceptor tyrosine-based inhibition motif (ITIM).

Protein type: Membrane protein, integral; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9p13-p12

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; SH2 domain binding; kinase binding

Biological Process: immune system process; signal transduction; regulation of T cell activation