Western Blot (WB)
(SIKE Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SIKE antibody detected the SIKE protein (arrow).)
Rabbit anti-Human SIKE Polyclonal Antibody | anti-SIKE antibody
SIKE, CT (SIKE1, SIKE, Suppressor of IKBKE 1, Suppressor of IKK-epsilon) (AP)
Gene Names
SIKE1; SIKE
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SIKE; Polyclonal Antibody; CT (SIKE1; Suppressor of IKBKE 1; Suppressor of IKK-epsilon) (AP); SIKE1; Suppressor of IKK-epsilon; anti-SIKE antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-SIKE antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SIKE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from the C-terminal region of human SIKE.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(SIKE Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SIKE antibody detected the SIKE protein (arrow).)
Western Blot (WB)
(SIKE Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SIKE antibody detected the SIKE protein (arrow).)
Immunohistochemistry (IHC)
(SIKE Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SIKE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SIKE Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SIKE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-SIKE antibody
SIKE interacts with IKK-epsilon (IKBKE; MIM 605048) and TBK1 (MIM 604834) and acts as a suppressor of TLR3 (MIM 603029) and virus-triggered interferon activation pathways (Huang et al., 2005 [PubMed 16281057]).
Product Categories/Family for anti-SIKE antibody
References
Goudreault, M., et al. Mol. Cell Proteomics 8(1):157-171(2009). Huang, J., et al. EMBO J. 24(23):4018-4028(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,261 Da
NCBI Official Full Name
suppressor of IKBKE 1 isoform 1
NCBI Official Synonym Full Names
suppressor of IKBKE 1
NCBI Official Symbol
SIKE1
NCBI Official Synonym Symbols
SIKE
NCBI Protein Information
suppressor of IKBKE 1
UniProt Protein Name
Suppressor of IKBKE 1
UniProt Gene Name
SIKE1
UniProt Synonym Gene Names
SIKE
NCBI Description
SIKE interacts with IKK-epsilon (IKBKE; MIM 605048) and TBK1 (MIM 604834) and acts as a suppressor of TLR3 (MIM 603029) and virus-triggered interferon activation pathways (Huang et al., 2005 [PubMed 16281057]).[supplied by OMIM, Mar 2008]
Uniprot Description
Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways.
Research Articles on SIKE
Similar Products
Product Notes
The SIKE sike1 (Catalog #AAA6347681) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIKE, CT (SIKE1, SIKE, Suppressor of IKBKE 1, Suppressor of IKK-epsilon) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIKE can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SIKE sike1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIKE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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