Collagen Type II, Bovine Articular Cartilage Protein | COL2A1 protein

Collagen Type II, Bovine Articular Cartilage

Cat. Num. AAA634576

• Gene Names: COL2A1; AOM; ANFH; SEDC; COL11A3
• Applications: ELISA
• Purity: ~99% (SDS-PAGE)
• Synonyms: Collagen Type II; Bovine Articular Cartilage; COL2A1 protein

• Host: Bovine articular cartilage
• Purity/Purification: ~99% (SDS-PAGE)
• Form/Format: Supplied as a lyophilized powder. Soluble in acidic buffer (< = 4mg/ml).

• Applicable Applications for COL2A1 protein: ELISA
• Grade: Highly Purified
• Solubility: Soluble in acidic buffer (maximum 4mg/ml), but difficult to dissolve in neutral buffer. In order to dissolve this product in neutral buffer, first dissolve in 0.01 or 0.05M acetic acid, and then add into neutral buffer containing 0.2M NaCl or dialyze against the same buffer.
• Related Products: Type I Collagen, ELISA Grade Type II Collagen ELISA Grade; Type II Collagen Adjuvant Type II Collagen ELISA Kits
• Note: To avoid fibril formation under neutral conditions, it is recommended to use a buffer containing 0.15-0.2M sodium chloride and keep on ice. Type II collagen does not form a stable gel with incubation at 37°C. To prepare a type II collagen gel, addition components such as Type I collagen or other substances may be required.
• Preparation and Storage: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Sensitive to light.; Shipping: Blue Ice

Related Product Information for COL2A1 protein

Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.

Highly purified type II collagen. Suitable for use as a standard for collagen analysis by SDS gel; as a substrate for MMP-1, MMP-8 and MMP-13; and as an antigen for immunizing animals for the induction of collagen-induced arthritis (CIA) in certain species and strains of experimental animals.

Product Categories/Family for COL2A1 protein

Molecular Biology; MB-Collagen

NCBI and Uniprot Product Information

• NCBI GI #: 119578374
• NCBI GeneID: 1280
• UniProt Accession #: P02458; Q12985; Q14009; Q14044; Q14045; Q14046; Q14047; Q14056; Q14058; Q16672; Q1JQ82; A6NGA0
• Molecular Weight: 300kD
• NCBI Official Full Name: collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital), isoform CRA_e
• NCBI Official Synonym Full Names: collagen, type II, alpha 1
• NCBI Official Symbol: COL2A1
• NCBI Official Synonym Symbols: AOM; ANFH; SEDC; COL11A3
• NCBI Protein Information: collagen alpha-1(II) chain; collagen alpha-1(II) chain; chondrocalcin; cartilage collagen; alpha-1 type II collagen; collagen II, alpha-1 polypeptide
• UniProt Protein Name: Collagen alpha-1(II) chain
• UniProt Gene Name: COL2A1
• UniProt Entry Name: CO2A1_HUMAN

NCBI Description

This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

COL2A1: the alpha-1 chain of type II collagen, an extra-cellular matrix protein found in cartilage and the vitreous humor of the eye. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Chondrocalcin is the calcium binding C-propeptide of this collagen molecule. Defects in this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. There are two transcripts identified for this gene. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Extracellular matrix

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; collagen type II; extracellular region; basement membrane

Molecular Function: identical protein binding; platelet-derived growth factor binding; metal ion binding; extracellular matrix structural constituent conferring tensile strength

Biological Process: heart morphogenesis; proteoglycan metabolic process; axon guidance; inner ear morphogenesis; extracellular matrix organization and biogenesis; collagen fibril organization; central nervous system development; palate development; notochord development; extracellular matrix disassembly; limb bud formation; collagen catabolic process; sensory perception of sound; visual perception; regulation of gene expression; cartilage development; tissue homeostasis; chondrocyte differentiation; skeletal development; endochondral ossification; cartilage condensation

Disease: Platyspondylic Lethal Skeletal Dysplasia, Torrance Type; Achondrogenesis, Type Ii; Kniest Dysplasia; Otospondylomegaepiphyseal Dysplasia; Spondyloepimetaphyseal Dysplasia, Strudwick Type; Avascular Necrosis Of Femoral Head, Primary; Spondyloepiphyseal Dysplasia Congenita; Legg-calve-perthes Disease; Spondyloperipheral Dysplasia; Epiphyseal Dysplasia, Multiple, With Myopia And Conductive Deafness; Osteoarthritis With Mild Chondrodysplasia; Stickler Syndrome, Type I; Czech Dysplasia; Stickler Syndrome, Type I, Nonsyndromic Ocular