Platelet Factor 4 Active Protein | PF4 active protein

Platelet Factor 4, Human (PF4, PF-4, C-X-C Motif Chemokine 4, CXCL4, Iroplact, Oncostatin-A, SCYB4)

Cat. Num. AAA634523

• Gene Names: PF4; PF-4; CXCL4; SCYB4
• Purity: Highly Purified; ~95% (SDS-PAGE, RP-HPLC)
• Synonyms: Platelet Factor 4; Human (PF4; PF-4; C-X-C Motif Chemokine 4; CXCL4; Iroplact; Oncostatin-A; SCYB4); PF4 active protein

• Purity/Purification: Highly Purified; ~95% (SDS-PAGE, RP-HPLC)
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with sterile dH2O to 100ug/ml.
• Sequence: The sequence of the first four N-terminal aa was determined and was found to be Glu-Ala-Glu-Glu.

• Biological Activity: Binding of HIT-antibodies
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for PF4 active protein

Platelet factor-4 is a 70aa protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin-A is a member of the CXC chemokine family. Human PF4 is used for the proof of heparin-induced thrombocytopenia. Furthermore it is used as an inhibitor in the angiogenesis during tumor therapy. Human PF-4 a 7.8kD protein consisting of 70aa residues.

Product Categories/Family for PF4 active protein

Molecular Biology; MB-Coagulation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4505733
• NCBI GeneID: 5196
• NCBI Accession #: NP_002610.1
• NCBI GenBank Nucleotide #: NM_002619.3
• UniProt Accession #: P02776; Q53X61; Q9UC64; Q9UC65
• Molecular Weight: ~7.8kD
• NCBI Official Full Name: platelet factor 4
• NCBI Official Synonym Full Names: platelet factor 4
• NCBI Official Symbol: PF4
• NCBI Official Synonym Symbols: PF-4; CXCL4; SCYB4
• NCBI Protein Information: platelet factor 4; iroplact; oncostatin-A; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4
• UniProt Protein Name: Platelet factor 4
• Protein Family: Platelet factor
• UniProt Gene Name: PF4
• UniProt Synonym Gene Names: CXCL4; SCYB4; PF-4
• UniProt Entry Name: PLF4_HUMAN

NCBI Description

This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. [provided by RefSeq, Mar 2012]

Uniprot Description

PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine; Apoptosis; Secreted

Chromosomal Location of Human Ortholog: 4q12-q21

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity

Biological Process: negative regulation of cytolysis; platelet activation; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; positive regulation of tumor necrosis factor production; negative regulation of megakaryocyte differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; platelet degranulation; negative regulation of MHC class II biosynthetic process; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of macrophage differentiation; blood coagulation; inflammatory response