Rabbit anti-Human SAMM50 Polyclonal Antibody | anti-SAMM50 antibody

SAMM50, ID (SAMM50, Sorting and assembly machinery component 50 homolog, Transformation-related gene 3 protein) (PE)

Cat. Num. AAA6344974

• Gene Names: SAMM50; OMP85; SAM50; TOB55; TRG-3; CGI-51; YNL026W
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SAMM50; Polyclonal Antibody; ID (SAMM50; Sorting and assembly machinery component 50 homolog; Transformation-related gene 3 protein) (PE); Transformation-related gene 3 protein; anti-SAMM50 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SAMM50 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SAMM50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-189 amino acids from the Central region of human SAMM50.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SAMM50 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SAMM50 antibody detected the SAMM50 protein (arrow).)

Related Product Information for anti-SAMM50 antibody

SAMM50 is a component of the sorting and assembly machinery (SAM) complex of the outer mitochondrial membrane. The SAM complex has a role in integrating beta-barrel proteins into the outer mitochondrial membrane (Humphries et al., 2005 [PubMed 15644312]).

Product Categories/Family for anti-SAMM50 antibody

Antibodies; Mitochondrial Proteins

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Yuan, X., et al. Am. J. Hum. Genet. 83(4):520-528(2008). Xie, J., et al. FEBS Lett. 581(18):3545-3549(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 225543166
• NCBI GeneID: 25813
• NCBI Accession #: NP_056195.3
• NCBI GenBank Nucleotide #: NM_015380.4
• UniProt Accession #: Q9Y512; Q53HC4; Q56VW7; Q969Y9; Q96I46; Q9NW85; Q9UQM9
• Molecular Weight: 51,976 Da
• NCBI Official Full Name: sorting and assembly machinery component 50 homolog
• NCBI Official Synonym Full Names: SAMM50 sorting and assembly machinery component
• NCBI Official Symbol: SAMM50
• NCBI Official Synonym Symbols: OMP85; SAM50; TOB55; TRG-3; CGI-51; YNL026W
• NCBI Protein Information: sorting and assembly machinery component 50 homolog; sorting and assembly machinery 50kDa; transformation-related gene 3 protein
• UniProt Protein Name: Sorting and assembly machinery component 50 homolog
• Protein Family: Sorting and assembly machinery component
• UniProt Gene Name: SAMM50
• UniProt Synonym Gene Names: SAM50; TRG-3
• UniProt Entry Name: SAM50_HUMAN

Uniprot Description

SAMM50: May be required for the assembly pathway of mitochondrial outer membrane proteins. Belongs to the SAM50/omp85 family.

Protein type: Membrane protein, multi-pass; Mitochondrial

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: mitochondrial outer membrane; mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: protein binding

Biological Process: cellular protein metabolic process; protein targeting to mitochondrion; protein import into mitochondrial outer membrane