Rabbit anti-Human RREB1 Polyclonal Antibody | anti-RREB1 antibody

RREB1, CT (RREB1, FINB, Ras-responsive element-binding protein 1, Finger protein in nuclear bodies, Raf-responsive zinc finger protein LZ321, Zinc finger motif enhancer-binding protein 1) (Biotin)

Cat. Num. AAA6344086

• Gene Names: RREB1; HNT; FINB; LZ321; Zep-1; RREB-1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RREB1; Polyclonal Antibody; CT (RREB1; FINB; Ras-responsive element-binding protein 1; Finger protein in nuclear bodies; Raf-responsive zinc finger protein LZ321; Zinc finger motif enhancer-binding protein 1) (Biotin); Zinc finger motif enhancer-binding protein 1; anti-RREB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RREB1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RREB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1398-1424 amino acids from the C-terminal region of human RREB1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RREB1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RREB1 antibody detected the RREB1 protein (arrow).)

Related Product Information for anti-RREB1 antibody

The protein encoded by this gene is a zinc finger transcription factor that binds to RAS-responsive elements (RREs) of gene promoters. It has been shown that the calcitonin gene promoter contains an RRE and that the encoded protein binds there and increases expression of calcitonin, which may be involved in Ras/Raf-mediated cell differentiation. Multiple transcript variants encoding several different isoforms have been found for this gene.

Product Categories/Family for anti-RREB1 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Neale, B.M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(16):7395-7400(2010). Milon, B.C., et al. Prostate 70(3):288-296(2010). Flajollet, S., et al. J. Immunol. 183(11):6948-6959(2009). Liu, H., et al. Biochem. J. 422(3):543-551(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 51173735
• NCBI GeneID: 6239
• NCBI Accession #: NP_001003698.1
• NCBI GenBank Nucleotide #: NM_001003698.3
• UniProt Accession #: Q92766; O75567; O75568; Q5VYB2; Q6BEP5; Q6BEP6; Q6BEP8; Q86SU2; Q9Y474; A2RRF5
• Molecular Weight: 181,420 Da
• NCBI Official Full Name: ras-responsive element-binding protein 1 isoform 2
• NCBI Official Synonym Full Names: ras responsive element binding protein 1
• NCBI Official Symbol: RREB1
• NCBI Official Synonym Symbols: HNT; FINB; LZ321; Zep-1; RREB-1
• NCBI Protein Information: ras-responsive element-binding protein 1; hindsight homolog; DNA-binding protein; finger protein in nuclear bodies; raf-responsive zinc finger protein LZ321; zinc finger motif enhancer binding protein 1; zinc finger motif enhancer-binding protein 1; zinc
• UniProt Protein Name: Ras-responsive element-binding protein 1
• Protein Family: Ras-responsive element-binding protein
• UniProt Gene Name: RREB1
• UniProt Synonym Gene Names: FINB; RREB-1; Zep-1
• UniProt Entry Name: RREB1_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger transcription factor that binds to RAS-responsive elements (RREs) of gene promoters. It has been shown that the calcitonin gene promoter contains an RRE and that the encoded protein binds there and increases expression of calcitonin, which may be involved in Ras/Raf-mediated cell differentiation. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

RREB1: Transcription factor that binds specifically to the RAS- responsive elements (RRE) of gene promoters. May be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. Represses the angiotensinogen gene. Negatively regulates the transcriptional activity of AR. Potentiates the transcriptional activity of NEUROD1. Belongs to the krueppel C2H2-type zinc-finger protein family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription, coactivator/corepressor; C2H2-type zinc finger protein; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: nucleoplasm; nuclear body; cytoplasm; nucleolus; nuclear speck; nucleus

Molecular Function: metal ion binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription, DNA-dependent; Ras protein signal transduction; positive regulation of mammary gland epithelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter