Rabbit anti-Human RRBP1 Polyclonal Antibody | anti-RRBP1 antibody

RRBP1, CT (RRBP1, KIAA1398, Ribosome-binding protein 1, 180kD ribosome receptor homolog, ES/130-related protein, Ribosome receptor protein) (Biotin)

Cat. Num. AAA6344075

• Gene Names: RRBP1; RRp; hES; ES130; ES/130
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RRBP1; Polyclonal Antibody; CT (RRBP1; KIAA1398; Ribosome-binding protein 1; 180kD ribosome receptor homolog; ES/130-related protein; Ribosome receptor protein) (Biotin); Ribosome receptor protein; anti-RRBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RRBP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RRBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1326-1355 amino acids from the C-terminal region of human RRBP1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RRBP1 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RRBP1 antibody detected the RRBP1 protein (arrow).)

Related Product Information for anti-RRBP1 antibody

Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants.

Product Categories/Family for anti-RRBP1 antibody

Antibodies; Binding Proteins

References

Ueno, T., et al. J. Biol. Chem. 285(39):29941-29950(2010). Ueno, T., et al. Exp. Cell Res. 316(3):329-340(2010). Krasnov, G.S., et al. Mol. Biol. (Mosk.) 43(2):348-356(2009). Benyamini, P., et al. Mol. Biol. Cell 20(2):732-744(2009). Bai, J.Z., et al. Cell Biol. Int. 32(5):473-483(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 110611220
• NCBI GeneID: 6238
• NCBI Accession #: NP_004578.2
• NCBI GenBank Nucleotide #: NM_004587.2
• UniProt Accession #: Q9P2E9; O75300; O75301; Q5W165; Q96SB2; Q9BWP1; Q9H476; A2A2S6; A6NCN6
• Molecular Weight: 108,632 Da
• NCBI Official Full Name: ribosome-binding protein 1
• NCBI Official Synonym Full Names: ribosome binding protein 1
• NCBI Official Symbol: RRBP1
• NCBI Official Synonym Symbols: RRp; hES; ES130; ES/130
• NCBI Protein Information: ribosome-binding protein 1; 180 kDa ribosome receptor homolog; ES/130-related protein; ribosome binding protein 1 homolog 180kDa (dog); ribosome receptor protein
• UniProt Protein Name: Ribosome-binding protein 1
• Protein Family: Ribosome-binding protein
• UniProt Gene Name: RRBP1
• UniProt Synonym Gene Names: KIAA1398; RRp
• UniProt Entry Name: RRBP1_HUMAN

NCBI Description

This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12. [provided by RefSeq, Apr 2012]