Rabbit anti-Human RPN1 Polyclonal Antibody | anti-RPN1 antibody

RPN1, CT (RPN1, Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67kD subunit, Ribophorin I, Ribophorin-1) (PE)

Cat. Num. AAA6343654

• Gene Names: RPN1; OST1; RBPH1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPN1; Polyclonal Antibody; CT (RPN1; Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 1; Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67kD subunit; Ribophorin I; Ribophorin-1) (PE); Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67kD subunit; Ribophorin-1; anti-RPN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RPN1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 558-587 amino acids from the C-terminal region of human RPN1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RPN1 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the RPN1 antibody detected the RPN1 protein (arrow).)

Related Product Information for anti-RPN1 antibody

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.

Product Categories/Family for anti-RPN1 antibody

Antibodies; Enzymes

References

Ruiz-Canada, C., et al. Cell 136(2):272-283(2009). Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008). Wilson, C.M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(28):9534-9539(2008). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4506675
• NCBI GeneID: 6184
• NCBI Accession #: NP_002941.1
• NCBI GenBank Nucleotide #: NM_002950.3
• UniProt Accession #: P04843; Q68DT1; B2R5Z0; D3DNB6
• Molecular Weight: Predicted MW: 68kDa
• NCBI Official Full Name: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
• NCBI Official Synonym Full Names: ribophorin I
• NCBI Official Symbol: RPN1
• NCBI Official Synonym Symbols: OST1; RBPH1
• NCBI Protein Information: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
• UniProt Protein Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
• Protein Family: 26S proteasome regulatory
• UniProt Gene Name: RPN1
• UniProt Synonym Gene Names: RPN-I
• UniProt Entry Name: RPN1_HUMAN

NCBI Description

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]