Rabbit anti-Human RNASEH2B Polyclonal Antibody | anti-RNASEH2B antibody
RNASEH2B, ID (RNASEH2B, DLEU8, Ribonuclease H2 subunit B, Aicardi-Goutieres syndrome 2 protein, Deleted in lymphocytic leukemia 8, Ribonuclease HI subunit B) (Azide free) (HRP)
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of RNASEH2B Antibody (Center) in CEM cell line lysates (35ug/lane). RNASEH2B (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(RNASEH2B Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RNASEH2B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
RNase H2 is composed of a single catalytic subunit (A) and two non-catalytic subunits (B and C) and specifically degrades the RNA of RNA:DNA hybrids. The protein encoded by this gene is the non-catalytic B subunit of RNase H2, which is thought to play a role in DNA replication. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Aicardi-Goutieres syndrome type 2 (AGS2). [provided by RefSeq, Nov 2008]
Uniprot Description
RNASEH2B: Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging- strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2). A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood. Belongs to the RNase H2 subunit B family.
Protein type: DNA replication; RNA processing
Chromosomal Location of Human Ortholog: 13q14.3
Cellular Component: nucleoplasm; nucleus
Molecular Function: ribonuclease H activity
Biological Process: positive regulation of fibroblast proliferation; ribonucleotide metabolic process; in utero embryonic development; RNA catabolic process; regulation of G2/M transition of mitotic cell cycle
Disease: Aicardi-goutieres Syndrome 2