Rabbit anti-Human RAPGEF5 Polyclonal Antibody | anti-RAPGEF5 antibody

RAPGEF5, CT (RAPGEF5, GFR, KIAA0277, MRGEF, Rap guanine nucleotide exchange factor 5, Guanine nucleotide exchange factor for Rap1, M-Ras-regulated Rap GEF, Related to Epac) (Azide free) (HRP)

Cat. Num. AAA6340299

• Gene Names: RAPGEF5; GFR; REPAC; MR-GEF
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAPGEF5; Polyclonal Antibody; CT (RAPGEF5; GFR; KIAA0277; MRGEF; Rap guanine nucleotide exchange factor 5; Guanine nucleotide exchange factor for Rap1; M-Ras-regulated Rap GEF; Related to Epac) (Azide free) (HRP); Related to Epac; anti-RAPGEF5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RAPGEF5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RAPGEF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 528-555 amino acids from the C-terminal region of human RAPGEF5.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RAPGEF5 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the RAPGEF5 antibody detected the RAPGEF5 protein (arrow).)

Related Product Information for anti-RAPGEF5 antibody

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP-and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).

Product Categories/Family for anti-RAPGEF5 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Okada, Y., et al. Hum. Mol. Genet. 19(11):2303-2312(2010). Hwang, S.J., et al. BMC Med. Genet. 8 SUPPL 1, S10 (2007): Rebhun, J.F., et al. J. Biol. Chem. 275(45):34901-34908(2000). de Rooij, J., et al. J. Biol. Chem. 275(27):20829-20836(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 119392077
• NCBI GeneID: 9771
• NCBI Accession #: NP_036426.3
• NCBI GenBank Nucleotide #: NM_012294.3
• UniProt Accession #: Q92565; Q8IXU5; A4D140
• Molecular Weight: 67,733 Da
• NCBI Official Full Name: rap guanine nucleotide exchange factor 5
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor (GEF) 5
• NCBI Official Symbol: RAPGEF5
• NCBI Official Synonym Symbols: GFR; REPAC; MR-GEF
• NCBI Protein Information: rap guanine nucleotide exchange factor 5; related to Epac; M-Ras-regulated GEF; M-Ras-regulated Rap GEF; guanine nucleotide exchange factor for Rap1
• UniProt Protein Name: Rap guanine nucleotide exchange factor 5
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: RAPGEF5
• UniProt Synonym Gene Names: GFR; KIAA0277; MRGEF; MR-GEF; Repac
• UniProt Entry Name: RPGF5_HUMAN

NCBI Description

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]

Uniprot Description

RAPGEF5: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: nucleus

Molecular Function: Rap guanyl-nucleotide exchange factor activity; GTP-dependent protein binding; Ras guanyl-nucleotide exchange factor activity

Biological Process: nervous system development; small GTPase mediated signal transduction