Western Blot (WB)
(RANGAP1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RANGAP1 antibody detected the RANGAP1 protein (arrow).)
Rabbit anti-Human RANGAP1 Polyclonal Antibody | anti-RANGAP1 antibody
RANGAP1, NT (RANGAP1, KIAA1835, SD, Ran GTPase-activating protein 1) (FITC)
Gene Names
RANGAP1; SD; Fug1; KIAA1835; MGC20266; RANGAP1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RANGAP1; Polyclonal Antibody; NT (RANGAP1; KIAA1835; SD; Ran GTPase-activating protein 1) (FITC); Ran GTPase-activating protein 1; anti-RANGAP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-RANGAP1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
RANGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human RANGAP1.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(RANGAP1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RANGAP1 antibody detected the RANGAP1 protein (arrow).)
Western Blot (WB)
(RANGAP1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RANGAP1 antibody detected the RANGAP1 protein (arrow).)
Western Blot (WB)
(Western blot analysis of RANGAP1 (arrow) using rabbit polyclonal RANGAP1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RANGAP1 gene.)
Western Blot (WB)
(Western blot analysis of RANGAP1 (arrow) using rabbit polyclonal RANGAP1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RANGAP1 gene.)
Immunohistochemistry (IHC)
(RANGAP1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RANGAP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(RANGAP1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RANGAP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-RANGAP1 antibody
RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.
Product Categories/Family for anti-RANGAP1 antibody
References
Zhang, J., et al. Biochem. Biophys. Res. Commun. 375(2):252-255(2008). Zuccolo, M., et al. EMBO J. 26(7):1853-1864(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Vertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006). Vertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
ran GTPase-activating protein 1 [Homo sapiens]
NCBI Official Synonym Full Names
Ran GTPase activating protein 1
NCBI Official Symbol
RANGAP1
NCBI Official Synonym Symbols
SD; Fug1; KIAA1835; MGC20266; RANGAP1
UniProt Protein Name
Ran GTPase-activating protein 1
Protein Family
UniProt Gene Name
RANGAP1
UniProt Synonym Gene Names
KIAA1835; SD
UniProt Entry Name
RAGP1_HUMAN
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Product Notes
The RANGAP1 rangap1 (Catalog #AAA6340232) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RANGAP1, NT (RANGAP1, KIAA1835, SD, Ran GTPase-activating protein 1) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RANGAP1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RANGAP1 rangap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RANGAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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