Rabbit anti-Human RAB21 Polyclonal Antibody | anti-RAB21 antibody

RAB21, CT (RAB21, KIAA0118, Ras-related protein Rab-21) (AP)

Cat. Num. AAA6339602

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAB21; Polyclonal Antibody; CT (RAB21; KIAA0118; Ras-related protein Rab-21) (AP); Ras-related protein Rab-21; anti-RAB21 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RAB21 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RAB21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 167-197 amino acids from the C-terminal region of human RAB21.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RAB21 Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the RAB21 antibody detected the RAB21 protein (arrow).)

Immunohistochemistry (IHC)

(RAB21 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RAB21 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-RAB21 antibody

RAB21 belongs to the RAB family of small GTP-binding proteins that regulate intracellular vesicle targeting (Opdam et al., 2000 [PubMed 10887961]).

Product Categories/Family for anti-RAB21 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Burgo, A., et al. EMBO Rep. 10(10):1117-1124(2009). Pellinen, T., et al. Dev. Cell 15(3):371-385(2008). Delprato, A., et al. Nat. Struct. Mol. Biol. 14(5):406-412(2007). Pellinen, T., et al. J. Cell Biol. 173(5):767-780(2006). Eathiraj, S., et al. Nature 436(7049):415-419(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 7661922
• NCBI GeneID: 23011
• NCBI Accession #: NP_055814.1
• NCBI GenBank Nucleotide #: NM_014999.2
• UniProt Accession #: Q9UL25; Q14466; Q569H3
• Molecular Weight: 24,348 Da
• NCBI Official Full Name: ras-related protein Rab-21
• NCBI Official Synonym Full Names: RAB21, member RAS oncogene family
• NCBI Official Symbol: RAB21
• NCBI Protein Information: ras-related protein Rab-21
• UniProt Protein Name: Ras-related protein Rab-21
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB21
• UniProt Synonym Gene Names: KIAA0118
• UniProt Entry Name: RAB21_HUMAN

NCBI Description

This gene belongs to the Rab family of monomeric GTPases, which are involved in the control of cellular membrane traffic. The encoded protein plays a role in the targeted trafficking of integrins via its association with integrin alpha tails. As a consequence, the encoded protein is involved in the regulation of cell adhesion and migration. [provided by RefSeq, Aug 2013]

Uniprot Description

RAB21: Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration. During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 12q21.1

Cellular Component: endoplasmic reticulum membrane; internal side of plasma membrane; focal adhesion; cytoplasmic vesicle membrane; early endosome; vesicle membrane; trans-Golgi network; endosome; cleavage furrow

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: regulation of exocytosis; intracellular protein transport; metabolic process; regulation of endocytosis; Rab protein signal transduction; positive regulation of receptor-mediated endocytosis