Rabbit anti-Human PSMB5 Polyclonal Antibody | anti-PSMB5 antibody

PSMB5, CT (PSMB5, LMPX, MB1, X, Proteasome subunit beta type-5, Macropain epsilon chain, Multicatalytic endopeptidase complex epsilon chain, Proteasome chain 6, Proteasome epsilon chain, Proteasome subunit MB1, Proteasome subunit X) (FITC)

Cat. Num. AAA6338197

• Gene Names: PSMB5; X; MB1; LMPX
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSMB5; Polyclonal Antibody; CT (PSMB5; LMPX; MB1; X; Proteasome subunit beta type-5; Macropain epsilon chain; Multicatalytic endopeptidase complex epsilon chain; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit MB1; Proteasome subunit X) (FITC); Proteasome subunit X; anti-PSMB5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PSMB5 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PSMB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-263 amino acids from the C-terminal region of human PSMB5.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PSMB5 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PSMB5 antibody detected the PSMB5 protein (arrow).)

Western Blot (WB)

(Western blot analysis of PSMB5 (arrow) using rabbit polyclonal PSMB5 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PSMB5 gene.)

Immunohistochemistry (IHC)

(PSMB5 Antibody (C-term) (AP14528b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PSMB5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PSMB5 antibody

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-PSMB5 antibody

Antibodies; Proteasomes

References

Ri, M., et al. Leukemia 24(8):1506-1512(2010). Lu, S., et al. Exp. Hematol. 37(7):831-837(2009). Lu, S., et al. Exp. Hematol. 36(10):1278-1284(2008). Oerlemans, R., et al. Blood 112(6):2489-2499(2008). Wang, L., et al. Clin. Cancer Res. 14(11):3503-3513(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 195539356
• NCBI GeneID: 5693
• NCBI Accession #: NP_001124197.1; NP_002788.1
• NCBI GenBank Nucleotide #: NM_001130725.1
• UniProt Accession #: P28074; Q16242; Q6PEW2; Q7Z3B5; Q86T01; Q9TNN9; B2R4N9; B4DUM9; D3DS43; E9PAV2
• Molecular Weight: 17,782 Da
• NCBI Official Full Name: proteasome subunit beta type-5 isoform 2
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, beta type, 5
• NCBI Official Symbol: PSMB5
• NCBI Official Synonym Symbols: X; MB1; LMPX
• NCBI Protein Information: proteasome subunit beta type-5; PSX large multifunctional protease X; macropain epsilon chain; multicatalytic endopeptidase complex epsilon chain; proteasome beta 5 subunit; proteasome catalytic subunit 3; proteasome chain 6; proteasome epsilon chain; pro
• UniProt Protein Name: Proteasome subunit beta type-5
• Protein Family: Proteasome
• UniProt Gene Name: PSMB5
• UniProt Synonym Gene Names: LMPX; MB1; X
• UniProt Entry Name: PSB5_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

PSMB5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.25.1; Proteasome complex

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; centrosome; nucleus; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; response to oxidative stress; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle