Rabbit anti-Human PRSS1 Polyclonal Antibody | anti-PRSS1 antibody

PRSS1, ID (PRSS1, TRP1, TRY1, TRYP1, Trypsin-1, Beta-trypsin, Cationic trypsinogen, Serine protease 1, Trypsin I, Alpha-trypsin chain 1, Alpha-trypsin chain 2) (PE)

Cat. Num. AAA6337896

• Gene Names: PRSS1; TRP1; TRY1; TRY4; TRYP1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRSS1; Polyclonal Antibody; ID (PRSS1; TRP1; TRY1; TRYP1; Trypsin-1; Beta-trypsin; Cationic trypsinogen; Serine protease 1; Trypsin I; Alpha-trypsin chain 1; Alpha-trypsin chain 2) (PE); Alpha-trypsin chain 2; anti-PRSS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PRSS1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PRSS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-111 amino acids from the Central region of human PRSS1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PRSS1 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PRSS1 antibody detected the PRSS1 protein (arrow).)

Related Product Information for anti-PRSS1 antibody

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7.

Product Categories/Family for anti-PRSS1 antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

Joergensen, M.T., et al. Am. J. Gastroenterol. 105(8):1876-1883(2010). Gasiorowska, A., et al. Dig. Dis. Sci. (2010). In press: Szmola, R., et al. J. Med. Genet. 47(5):348-350(2010). Nakanuma, S., et al. Int. J. Oncol. 36(4):793-800(2010). Johansen, D., et al. Pancreatology 10 (2-3), 229-237 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4506145
• NCBI GeneID: 5644
• NCBI Accession #: NP_002760.1
• NCBI GenBank Nucleotide #: NM_002769.4
• UniProt Accession #: P07477; Q5NV57; Q7M4N3; Q7M4N4; Q92955; Q9HAN4; Q9HAN5; Q9HAN6; Q9HAN7; A1A509; A6NJ71; B2R5I5
• Molecular Weight: 160,000
• NCBI Official Full Name: trypsin-1 preproprotein
• NCBI Official Synonym Full Names: protease, serine, 1 (trypsin 1)
• NCBI Official Symbol: PRSS1
• NCBI Official Synonym Symbols: TRP1; TRY1; TRY4; TRYP1
• NCBI Protein Information: trypsin-1; beta-trypsin; trypsinogen 1; trypsinogen A; digestive zymogen; cationic trypsinogen; nonfunctional trypsin 1
• UniProt Protein Name: Trypsin-1
• Protein Family: Anionic trypsin
• UniProt Gene Name: PRSS1
• UniProt Synonym Gene Names: TRP1; TRY1; TRYP1
• UniProt Entry Name: TRY1_HUMAN

NCBI Description

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. [provided by RefSeq, Jul 2008]

Uniprot Description

trypsin 1: Has activity against the synthetic substrates Boc-Phe- Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val- Pro-Arg-Mec. The single-chain form is more active than the two- chain form against all of these substrates. Defects in PRSS1 are a cause of pancreatitis (PCTT). A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Belongs to the peptidase S1 family.

Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.21.4

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: extracellular region

Molecular Function: metal ion binding; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; vitamin metabolic process; cobalamin metabolic process; digestion; proteolysis; water-soluble vitamin metabolic process

Disease: Trypsinogen Deficiency; Pancreatitis, Hereditary