Rabbit anti-Human, Mouse Prkaca Polyclonal Antibody | anti-Prkaca antibody

Prkaca, CT (Prkacb, Pkacb, cAMP-dependent protein kinase catalytic subunit beta) (APC)

Cat. Num. AAA6337238

• Gene Names: Prkacb; CbPKA; Pkacb
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Prkaca; Polyclonal Antibody; CT (Prkacb; Pkacb; cAMP-dependent protein kinase catalytic subunit beta) (APC); Prkacb; cAMP-dependent protein kinase catalytic subunit beta; anti-Prkaca antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 398

• Applicable Applications for anti-Prkaca antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Prkaca/Prkacb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-319 amino acids from the C-terminal region of Mouse Prkaca/Prkacb.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Prkaca/Prkacb Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Prkaca/Prkacb antibody detected the Prkaca/Prkacb protein (arrow).)

Western Blot (WB)

(Mouse Prkaca/Prkacb Antibody (C-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the Prkaca/Prkacb antibody detected the Prkaca/Prkacb protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Prkaca/Prkacb Antibody (C-term) (AP14456b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Prkaca/Prkacb Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Prkaca antibody

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.

Product Categories/Family for anti-Prkaca antibody

Antibodies; Protein Kinases

References

Ribeiro, R.A., et al. Br. J. Nutr. 104(8):1148-1155(2010). Liu, J.X., et al. Seizure 19(7):414-420(2010). Ha, C.H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(35):15467-15472(2010). Sarma, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):13165-13170(2010). Rah, S.Y., et al. J. Biol. Chem. 285(28):21877-21887(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 255958156
• NCBI GeneID: 18749
• NCBI Accession #: NP_001157672.1; NP_001157670.1
• NCBI GenBank Nucleotide #: NP_001157672.1
• UniProt Accession #: P68181
• NCBI Official Full Name: cAMP-dependent protein kinase catalytic subunit beta isoform 4
• NCBI Official Synonym Full Names: protein kinase, cAMP dependent, catalytic, beta
• NCBI Official Symbol: Prkacb
• NCBI Official Synonym Symbols: CbPKA; Pkacb
• NCBI Protein Information: cAMP-dependent protein kinase catalytic subunit beta
• UniProt Protein Name: cAMP-dependent protein kinase catalytic subunit beta
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: Prkacb
• UniProt Synonym Gene Names: Pkacb; PKA C-beta
• UniProt Entry Name: KAPCB_MOUSE

Uniprot Description

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis ().