Rabbit anti-Human PRDM14 Polyclonal Antibody | anti-PRDM14 antibody

PRDM14, NT (PRDM14, PR domain zinc finger protein 14, PR domain-containing protein 14) (Biotin)

Cat. Num. AAA6337052

• Gene Names: PRDM14; PFM11
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot, Immunofluorescence
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PRDM14; Polyclonal Antibody; NT (PRDM14; PR domain zinc finger protein 14; PR domain-containing protein 14) (Biotin); PR domain-containing protein 14; anti-PRDM14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PRDM14 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PRDM14 (PFM11) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human PRDM14.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PRDM14 polyclonal antibody in A549 cell lysate. PRDM14 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunofluorescence (IF)

(IFanalysis of PRDM14 in breast cancer cells. SKBr-3 cells were transfected with pCMV-Tag2A or pCMV-PRDM14 and then labeled with anti-PRDM14 antibody. The nucleus was stained with DAPI.)

Related Product Information for anti-PRDM14 antibody

Similar to acetylation and phosphorylation, histone methylation at the N terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET domain containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET domain family, and another 17 make up the PR domain family that is related to the SET domain family. PRDM14 is part of a family of PR domain genes that are involved in tumorigenesis. It may function as a transcription factor.

Product Categories/Family for anti-PRDM14 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Xiao, B., et al., Curr. Opin. Struct. Biol. 13(6):699-705 (2003). Dias Neto, E., et al., Proc. Natl. Acad. Sci. U.S.A. 97(7):3491-3496 (2000). Jiang, G.L., et al., Histol. Histopathol. 15(1):109-117 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 13375636
• NCBI GeneID: 63978
• NCBI Accession #: NP_078780.1
• NCBI GenBank Nucleotide #: NM_024504.3
• UniProt Accession #: Q9GZV8; Q86UX9
• Molecular Weight: 64,062 Da
• NCBI Official Full Name: PR domain zinc finger protein 14
• NCBI Official Synonym Full Names: PR/SET domain 14
• NCBI Official Symbol: PRDM14
• NCBI Official Synonym Symbols: PFM11
• NCBI Protein Information: PR domain zinc finger protein 14
• UniProt Protein Name: PR domain zinc finger protein 14
• Protein Family: PR domain zinc finger protein
• UniProt Gene Name: PRDM14

NCBI Description

This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer. [provided by RefSeq, Dec 2011]

Uniprot Description

PRDM14: a probable protein lysine methyltransferase that works in concert with the core human embryonic stem cell (ESC) regulators to control pluripotency associated genes. Binds to silenced genes in human ESCs. Its binding profile is enriched for the repressive tri-methylation of histone H3 lysine 27 (H3K27me3) modification. Interacts directly with the polycomb repressive complex 2 (PRC2). PRC2 is detected at PRDM14-bound loci in human ESCs. A probable transcriptional regulator of the krueppel C2H2-type zinc-finger protein family. Has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming. Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.

Protein type: C2H2-type zinc finger protein; Methyltransferase, protein arginine, predicted

Chromosomal Location of Human Ortholog: 8q13.3

Cellular Component: nucleoplasm

Molecular Function: chromatin DNA binding; metal ion binding; methyltransferase activity; protein binding; RNA binding

Biological Process: cell fate specification; cell morphogenesis; embryo implantation; fertilization; germ cell development; germ-line stem cell maintenance; homeostasis of number of cells within a tissue; inner cell mass cell fate commitment; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of flagellated sperm motility; somatic stem cell maintenance; transcription, DNA-dependent