Western Blot (WB)
(Western blot analysis of PRDM14 polyclonal antibody in A549 cell lysate. PRDM14 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human PRDM14 Polyclonal Antibody | anti-PRDM14 antibody
PRDM14 Antibody (N-term)
Western Blot (WB)
(Western blot analysis of PRDM14 polyclonal antibody in A549 cell lysate. PRDM14 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of PRDM14 polyclonal antibody in A549 cell lysate. PRDM14 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunofluorescence (IF)
(IFanalysis of PRDM14 in breast cancer cells. SKBr-3 cells were transfected with pCMV-Tag2A or pCMV-PRDM14 and then labeled with anti-PRDM14 antibody. The nucleus was stained with DAPI.)
Immunofluorescence (IF)
(IFanalysis of PRDM14 in breast cancer cells. SKBr-3 cells were transfected with pCMV-Tag2A or pCMV-PRDM14 and then labeled with anti-PRDM14 antibody. The nucleus was stained with DAPI.)
PRDM14 is part of a family of PR domain genes that are involved in tumorigenesis. It may function as a transcription factor.
Dias Neto, E., et al., Proc. Natl. Acad. Sci. U.S.A. 97(7):3491-3496 (2000).
Jiang, G.L., et al., Histol. Histopathol. 15(1):109-117 (2000).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer. [provided by RefSeq, Dec 2011]
Uniprot Description
PRDM14: a probable protein lysine methyltransferase that works in concert with the core human embryonic stem cell (ESC) regulators to control pluripotency associated genes. Binds to silenced genes in human ESCs. Its binding profile is enriched for the repressive tri-methylation of histone H3 lysine 27 (H3K27me3) modification. Interacts directly with the polycomb repressive complex 2 (PRC2). PRC2 is detected at PRDM14-bound loci in human ESCs. A probable transcriptional regulator of the krueppel C2H2-type zinc-finger protein family. Has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming. Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.
Protein type: C2H2-type zinc finger protein; Methyltransferase, protein arginine, predicted
Chromosomal Location of Human Ortholog: 8q13.3
Cellular Component: nucleoplasm
Molecular Function: methyltransferase activity; protein binding; DNA binding; metal ion binding
Biological Process: fertilization; transcription, DNA-dependent; regulation of transcription, DNA-dependent; somatic stem cell maintenance; homeostasis of number of cells within a tissue; negative regulation of fibroblast growth factor receptor signaling pathway; cell morphogenesis; inner cell mass cell fate commitment; germ-line stem cell maintenance; cell fate specification; regulation of gene expression, epigenetic; germ cell development
Research Articles on PRDM14
Similar Products
Product Notes
The PRDM14 prdm14 (Catalog #AAA9202275) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The PRDM14 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRDM14 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the PRDM14 prdm14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRDM14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
