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Western Blot (WB)
(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6002288. This demonstrates that MBS6002288 detected the PRAMEF6 protein (arrow).)
Rabbit anti-Human PRAMEF6 Polyclonal Antibody | anti-PRAMEF6 antibody
PRAMEF6, CT (PRAMEF6, PRAME family member 6) (APC)
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
PRAMEF6; Polyclonal Antibody; CT (PRAMEF6; PRAME family member 6) (APC); PRAME family member 6; anti-PRAMEF6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PRAMEF6.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-PRAMEF6 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 395-423 in the C-terminal region of human PRAMEF6, UniProt Accession #Q5VXH4; Q5TYX0; A6NMV5.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6002288. This demonstrates that MBS6002288 detected the PRAMEF6 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6002288. This demonstrates that MBS6002288 detected the PRAMEF6 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS6002288 followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS6002288 followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis with NCI-H460 cells using MBS6002288 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis with NCI-H460 cells using MBS6002288 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of NCI-H460 cells using MBS6002288 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of NCI-H460 cells using MBS6002288 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-PRAMEF6 antibody
References
Gregory, S.G., et al. Nature 441(7091):315-321(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,852 Da
NCBI Official Full Name
PRAME family member 6
NCBI Official Synonym Full Names
PRAME family member 6
NCBI Official Symbol
PRAMEF6
NCBI Protein Information
PRAME family member 6
UniProt Protein Name
PRAME family member 6
Protein Family
UniProt Gene Name
PRAMEF6
Uniprot Description
PRAMEF6: Belongs to the PRAME family.
Chromosomal Location of Human Ortholog: 1p36.21
Biological Process: negative regulation of apoptosis; negative regulation of cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation
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Product Notes
The PRAMEF6 pramef6 (Catalog #AAA6336985) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRAMEF6, CT (PRAMEF6, PRAME family member 6) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRAMEF6 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PRAMEF6 pramef6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRAMEF6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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