Rabbit anti-Human PODNL1 Polyclonal Antibody | anti-PODNL1 antibody

PODNL1, ID (PODNL1, SLRR5B, Podocan-like protein 1) (APC)

Cat. Num. AAA6335478

• Gene Names: PODNL1; SLRR5B
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PODNL1; Polyclonal Antibody; ID (PODNL1; SLRR5B; Podocan-like protein 1) (APC); Podocan-like protein 1; anti-PODNL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 512

• Applicable Applications for anti-PODNL1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PODNL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-195 amino acids from the Central region of human PODNL1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PODNL1 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PODNL1 antibody detected the PODNL1 protein (arrow).)

Immunohistochemistry (IHC)

(PODNL1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PODNL1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PODNL1 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PODNL1 antibody

Antibodies; Carbohydrates; Glycoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 40254971
• NCBI GeneID: 79883
• NCBI Accession #: NP_079101.2
• NCBI GenBank Nucleotide #: NP_079101.2
• UniProt Accession #: Q6PEZ8
• NCBI Official Full Name: podocan-like 1
• NCBI Official Synonym Full Names: podocan like 1
• NCBI Official Symbol: PODNL1
• NCBI Official Synonym Symbols: SLRR5B
• NCBI Protein Information: podocan-like protein 1
• UniProt Protein Name: Podocan-like protein 1
• Protein Family: Podocan-like protein
• UniProt Gene Name: PODNL1
• UniProt Synonym Gene Names: SLRR5B

Uniprot Description

PODNL1: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class V subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: cytoplasm; proteinaceous extracellular matrix

Molecular Function: protein kinase inhibitor activity

Biological Process: cytokine-mediated signaling pathway; negative regulation of JAK-STAT cascade; negative regulation of protein kinase activity