Rabbit PKC beta2 Polyclonal Antibody | anti-PKCB antibody

PKC beta2, CT (PRKCB, PKCB, PRKCB1, Protein kinase C beta type) (APC)

Cat. Num. AAA6334323

• Gene Names: PRKCB; PKCB; PRKCB1; PRKCB2; PKCI(2); PKCbeta; PKC-beta
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PKC beta2; Polyclonal Antibody; CT (PRKCB; PKCB; PRKCB1; Protein kinase C beta type) (APC); PRKCB; Protein kinase C beta type; anti-PKCB antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PKCB antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PKC beta2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 644~673 amino acids from the C-terminal region of human PKC beta2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(The anti-PKC beta2 Pab is used in Western blot to detect PKC beta2 in Jurkat cell lysate (lane 1) and mouse brain tissue lysate (lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC beta2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-PKCB antibody

Protein kinase C (PKC) is a family of serine-and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC beta is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Isoform 1 uses an alternate exon at the 3' end compared to isoform 2, which includes a part of the coding region. The resulting isoform 1 has a distinct and shorter C-terminus, as compared to isoform 2.

Product Categories/Family for anti-PKCB antibody

Antibodies; Protein Kinases

References

Chen, D., et al., J. Biol. Chem. 279(6):4829-4839 (2004). Hug, B.A., et al., J. Biol. Chem. 279(2):825-830 (2004). Wu, H., et al., Biochem. Biophys. Res. Commun. 311(4):948-953 (2003). Becker, K.P., et al., J. Biol. Chem. 278(52):52747-52754 (2003). Birikh, K.R., et al., Proc. Natl. Acad. Sci. U.S.A. 100(1):283-288 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 125544
• NCBI GeneID: 5579
• UniProt Accession #: P05127; P05771
• NCBI Official Synonym Full Names: protein kinase C beta
• NCBI Official Symbol: PRKCB
• NCBI Official Synonym Symbols: PKCB; PRKCB1; PRKCB2; PKCI(2); PKCbeta; PKC-beta
• NCBI Protein Information: protein kinase C beta type

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]