Rabbit anti-Human PIGN Polyclonal Antibody | anti-PIGN antibody

PIGN, NT (PIGN, MCD4, GPI ethanolamine phosphate transferase 1, MCD4 homolog, Phosphatidylinositol-glycan biosynthesis class N protein) (Azide free) (HRP)

Cat. Num. AAA6333864

• Gene Names: PIGN; MCD4; MDC4; MCAHS; PIG-N; MCAHS1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PIGN; Polyclonal Antibody; NT (PIGN; MCD4; GPI ethanolamine phosphate transferase 1; MCD4 homolog; Phosphatidylinositol-glycan biosynthesis class N protein) (Azide free) (HRP); Phosphatidylinositol-glycan biosynthesis class N protein; anti-PIGN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PIGN antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PIGN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213~243 amino acids from the N-terminal region of human PIGN.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PIGN Antibody (N-term) in mouse NIH-3T3 cell line lysates (35ug/lane). PIGN (arrow) was detected using the purified Pab.)

Related Product Information for anti-PIGN antibody

PIGN is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is expressed in the endoplasmic reticulum and transfers phosphoethanolamine (EtNP) to the first mannose of the GPI anchor.

Product Categories/Family for anti-PIGN antibody

Antibodies; Enzymes; Glycosyltransferase

References

McDonough, C.W., et al. Hum. Genet. (2009). In press: Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005). Kinoshita, T., et al. Curr Opin Chem Biol 4(6):632-638(2000). Hong, Y., et al. J. Biol. Chem. 274(49):35099-35106(1999). Gaynor, E.C., et al. Mol. Biol. Cell 10(3):627-648(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 6912500
• NCBI GeneID: 23556
• NCBI Accession #: NP_036459.1
• NCBI GenBank Nucleotide #: NM_012327.5
• UniProt Accession #: O95427; Q7L8F8; Q8TC01; Q9NT05
• Molecular Weight: 105,810 Da
• NCBI Official Full Name: GPI ethanolamine phosphate transferase 1
• NCBI Official Synonym Full Names: phosphatidylinositol glycan anchor biosynthesis class N
• NCBI Official Symbol: PIGN
• NCBI Official Synonym Symbols: MCD4; MDC4; MCAHS; PIG-N; MCAHS1
• NCBI Protein Information: GPI ethanolamine phosphate transferase 1
• UniProt Protein Name: GPI ethanolamine phosphate transferase 1
• UniProt Gene Name: PIGN
• UniProt Synonym Gene Names: MCD4; PIG-N

NCBI Description

This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is expressed in the endoplasmic reticulum and transfers phosphoethanolamine (EtNP) to the first mannose of the GPI anchor. Two alternatively spliced variants, which encode an identical isoform, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PIGN: Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor. Defects in PIGN are the cause of multiple congenital anomalies-hypotonia-seizures syndrome type 1 (MCAHS1). An autosomal recessive disorder characterized by neonatal hypotonia, lack of psychomotor development, seizures, dysmorphic features, and variable congenital anomalies involving the cardiac, urinary, and gastrointestinal systems. Most affected individuals die before 3 years of age. Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.

Protein type: EC 2.-.-.-; Endoplasmic reticulum; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Membrane protein, integral; Membrane protein, multi-pass; Transferase

Chromosomal Location of Human Ortholog: 18q21.33

Cellular Component: cytosol; endoplasmic reticulum membrane; integral component of membrane; membrane; plasma membrane

Molecular Function: mannose-ethanolamine phosphotransferase activity; phosphotransferase activity, for other substituted phosphate groups

Biological Process: GPI anchor biosynthetic process; preassembly of GPI anchor in ER membrane

Disease: Multiple Congenital Anomalies-hypotonia-seizures Syndrome 1