Mouse STAT 6 Monoclonal Antibody | anti-STAT6 antibody

STAT 6, phosphorylated (STAT6B, STAT6C, D12S1644, IL-4-STAT, interleukin 4 induced STAT, Signal Transducer and Activator of Transcription 6)

Cat. Num. AAA633383

• Gene Names: STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Highly Purified; Puri ed by subsequent thiophilic adsoption and size exclusion chromatography.
• Synonyms: STAT 6; Monoclonal Antibody; phosphorylated (STAT6B; STAT6C; D12S1644; IL-4-STAT; interleukin 4 induced STAT; Signal Transducer and Activator of Transcription 6); Anti -STAT 6; anti-STAT6 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4i61
• Specificity: Recognizes phosphorylated STAT6, Mr 100kD. Species Crossreactivity: Human, mouse and rat.
• Purity/Purification: Highly Purified; Puri ed by subsequent thiophilic adsoption and size exclusion chromatography.
• Form/Format: Supplied as a liquid in ~143ul of PBS, 0.09% sodium azide before the addition of glycerol to 30%.

• Applicable Applications for anti-STAT6 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Immunoblot.; Dilution: Immunoblot Analysis: 0.5-2ug/ml detects phospho-STAT 6 in RIPA lysates from IL4-stimulated HepG2 cells. Unstimulated (lane 1) or IL4 stimulated HepG2 (lane 2) cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-STAT 6 (Y641), 0.5ug/ml. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Immunogen: KLH-conjugated, synthetic phosphopeptide corresponding to amino acids 630-650 (KPEQMQKDQRQ[pY]VPATIKMTV) of human STAT 6. The immunizing sequence has 19/21 identical amino acids in mouse.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(WB: Unstimulated (lane 1) or IL4 stimulated HepG2 (lane 2) cell lysate was probed with MBS633383 (2μg/ml).)

Product Categories/Family for anti-STAT6 antibody

Antibodies; Abs to Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 296010866
• NCBI GeneID: 6778
• NCBI Accession #: NP_001171550.1
• NCBI GenBank Nucleotide #: NM_001178079.1
• UniProt Accession #: P42226; Q5FBW5; Q71UP4; A8K316; Q56TK0; Q5FBW6; Q6LCD8; Q9BQD2
• Molecular Weight: 94,135 Da
• NCBI Official Full Name: signal transducer and activator of transcription 6 isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 6, interleukin-4 induced
• NCBI Official Symbol: STAT6
• NCBI Official Synonym Symbols: STAT6B; STAT6C; D12S1644; IL-4-STAT
• NCBI Protein Information: signal transducer and activator of transcription 6; STAT, interleukin4-induced; transcription factor IL-4 STAT
• UniProt Protein Name: Signal transducer and activator of transcription 6
• Protein Family: Signal transducer and transcription activator
• UniProt Gene Name: STAT6
• UniProt Entry Name: STAT6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.

Uniprot Description

STAT6: transcription factor of the STAT family. Plays a central role in IL4-mediated biological responses. Induces the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. May function in the differentiation of T helper 2 cells and class switch of immunoglobulins. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; signal transducer activity; protein phosphatase binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; T-helper 1 cell lineage commitment; negative regulation of T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interferon type I production; innate immune response; mammary gland epithelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of isotype switching to IgE isotypes; regulation of cell proliferation