Rabbit anti-Human PIG3 Polyclonal Antibody | anti-PIG3 antibody

PIG3, CT (TP53I3, PIG3, Quinone oxidoreductase PIG3, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein) (APC)

Cat. Num. AAA6333795

• Gene Names: TP53I3; PIG3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PIG3; Polyclonal Antibody; CT (TP53I3; Quinone oxidoreductase PIG3; Tumor protein p53-inducible protein 3; p53-induced gene 3 protein) (APC); TP53I3; p53-induced gene 3 protein; anti-PIG3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 332

• Applicable Applications for anti-PIG3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PIG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-203 amino acids from the C-terminal region of human PIG3.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PIG3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PIG3 antibody detected the PIG3 protein (arrow).)

Related Product Information for anti-PIG3 antibody

The protein encoded by this gene is similar to oxidoreductases, which are enzymes involved in cellular responses to oxidative stresses and irradiation. This gene is induced by the tumor suppressor p53 and is thought to be involved in p53-mediated cell death. It contains a p53 consensus binding site in its promoter region and a downstream pentanucleotide microsatellite sequence. P53 has been shown to transcriptionally activate this gene by interacting with the downstream pentanucleotide microsatellite sequence. The microsatellite is polymorphic, with a varying number of pentanucleotide repeats directly correlated with the extent of transcriptional activation by p53. It has been suggested that the microsatellite polymorphism may be associated with differential susceptibility to cancer. At least two transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-PIG3 antibody

Antibodies; Enzymes; Reductase

References

Kotsinas, A., et al. Oncogene 29 (37), 5220 (2010): Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Lee, J.H., et al. Oncogene 29(10):1431-1450(2010). Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010). Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 22538444
• NCBI GeneID: 9540
• NCBI Accession #: NP_004872.2
• NCBI GenBank Nucleotide #: NP_004872.2
• UniProt Accession #: Q53FA7
• NCBI Official Full Name: quinone oxidoreductase PIG3 isoform 1
• NCBI Official Synonym Full Names: tumor protein p53 inducible protein 3
• NCBI Official Symbol: TP53I3
• NCBI Official Synonym Symbols: PIG3
• NCBI Protein Information: quinone oxidoreductase PIG3
• UniProt Protein Name: Quinone oxidoreductase PIG3
• UniProt Gene Name: TP53I3
• UniProt Synonym Gene Names: PIG3
• UniProt Entry Name: QORX_HUMAN

NCBI Description

The protein encoded by this gene is similar to oxidoreductases, which are enzymes involved in cellular responses to oxidative stresses and irradiation. This gene is induced by the tumor suppressor p53 and is thought to be involved in p53-mediated cell death. It contains a p53 consensus binding site in its promoter region and a downstream pentanucleotide microsatellite sequence. P53 has been shown to transcriptionally activate this gene by interacting with the downstream pentanucleotide microsatellite sequence. The microsatellite is polymorphic, with a varying number of pentanucleotide repeats directly correlated with the extent of transcriptional activation by p53. It has been suggested that the microsatellite polymorphism may be associated with differential susceptibility to cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

Uniprot Description

TP53I3: May be involved in the generation of reactive oxygen species (ROS). Has low NADPH-dependent beta-naphthoquinone reductase activity, with a preference for 1,2-beta-naphthoquinone over 1,4-beta-naphthoquinone. Has low NADPH-dependent diamine reductase activity (in vitro). Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.-.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p23.3

Molecular Function: protein homodimerization activity; zinc ion binding; NADPH:quinone reductase activity; quinone binding

Biological Process: NADP metabolic process