Mouse Papilloma Virus, Type 16, Early Protein 7 Monoclonal Antibody
Papilloma Virus, Human, Type 16, Early Protein 7 (hPV)
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Papilloma Virus; Type 16; Early Protein 7; Monoclonal Antibody; Human; Early Protein 7 (hPV); Anti -Papilloma Virus; anti-Papilloma Virus; Early Protein 7 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
5E733
Specificity
Recognizes E7 protein of HPV type 16. Crossreactive with E7 protein of HPV type 18.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Applicable Applications for anti-Papilloma Virus, Type 16, Early Protein 7 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot and ELISA.
Immunogen
HPV oncoprotein E7, type 16
Hybridoma
SP2/0 myeloma cells with spleen cells of Balb/c mice immunized with HPV oncoprotein E7, type 16.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Papilloma Virus, Type 16, Early Protein 7 antibody
Specific types of HPV have been shown to be associated with human cervical cancer. One of these types, HPV16, has been shown to be present in the majority of cervical dysplasias and squamous cell carcinomas studied. Research has also shown that the expression of the HPV16-E7 gene is both sufficient and necessary for cellular transformation in vitro. The immunological detection of HPV types in human samples has been hindered by the lack of an in vitro culture system for the propagation of viruses and viral antigens. Although antibodies that were generated against the bovine papillomavirus capsid antigen (BPV-L1 ORF) are cross-reactive to HPV, these antibodies are of limited use because they do not discriminate among HPV types and have limited sensitivity as compared to standard light microscopic criteria for HPV infection. With bacterial expression systems, fusion proteins of HPV-encoded gene products can be generated and these, in turn, used to develop HPV type specific antibodies. The use of these type specific antibodies to study HPV protein expression may expand understanding of the role of HPV proteins in the pathogenesis of condylomas, dysplasias, and carcinomas of the genital tract.
Product Categories/Family for anti-Papilloma Virus, Type 16, Early Protein 7 antibody
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Product Notes
The Papilloma Virus, Type 16, Early Protein 7 (Catalog #AAA633242) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Papilloma Virus, Type 16, Early Protein 7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot and ELISA. Researchers should empirically determine the suitability of the Papilloma Virus, Type 16, Early Protein 7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Papilloma Virus, Type 16, Early Protein 7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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