Mouse Tyrosine Monoclonal Antibody | anti-TH antibody

Tyrosine, phosphorylated (Tyr) (Agarose)

Cat. Num. AAA633139

• Gene Names: TH; TYH; DYT14; DYT5b
• Applications: ELISA, Immunoprecipitation
• Purity: Purified; Purified.
• Synonyms: Tyrosine; Monoclonal Antibody; phosphorylated (Tyr) (Agarose); Anti -Tyrosine; anti-TH antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3A45
• Specificity: Useful for immunoprecipitation of tyrosine-phosphorylated proteins. Immobilized via the conjugation of carbohydrates to cross-linked agarose hydrazide beads. Does not crossreact with proteins phosphorylated on serine or threonine.
• Purity/Purification: Purified; Purified.
• Form/Format: Supplied as a liquid in 1X Cell Lysis Buffer, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-TH antibody: ELISA (EL/EIA), Immunoprecipitation (IP)
• Application Notes: Dilution: Immunoprecipitation: 1:10; 20ul of 50% bead slurry to 200ul of cell lysate.
• Immunogen: Phospho-Tyr-containing peptides (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunoprecipitation (IP)

(Immunoprecipitation of NIH/3T3 cell extracts untreated and treated with sodium vanadate (1 mM for 0.5 hour), using Immobilized MBS633139 (P-Tyr-100), and then probed with Phospho-Tyrosine Monoclonal Antibody (P-Tyr-100))

Related Product Information for anti-TH antibody

Since the original observations that protein tyrosine kinases (PTKs) play critical roles in oncogenic transformation (1,2), it has become clear that both protein tyrosine kinases and phospho-tyrosine phosphatases control a wide range of critical biological processes (3,4). Antibodies specific for phospho-tyrosine (5,6) have been invaluable reagents in these studies. Exceptionally sensitive new tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

Product Categories/Family for anti-TH antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 88900505
• NCBI GeneID: 7054
• NCBI Accession #: NP_954987.2
• NCBI GenBank Nucleotide #: NM_199293.2
• UniProt Accession #: P07101; Q15585; Q15588; Q15589; B7ZL70
• Molecular Weight: 58,600 Da
• NCBI Official Full Name: tyrosine 3-monooxygenase isoform c
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: TH
• NCBI Official Synonym Symbols: TYH; DYT14; DYT5b
• NCBI Protein Information: tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase
• UniProt Protein Name: Tyrosine 3-monooxygenase
• Protein Family: Tyrosine permease
• UniProt Gene Name: TH
• UniProt Synonym Gene Names: TYH; TH
• UniProt Entry Name: TY3H_HUMAN

NCBI Description

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Oxidoreductase; Vesicle; Amino Acid Metabolism - tyrosine; Mitochondrial; EC 1.14.16.2; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: neuron projection; internal side of plasma membrane; smooth endoplasmic reticulum; mitochondrion; melanosome membrane; dendrite; terminal button; perikaryon; cytosol; synaptic vesicle; cytoplasm; cytoplasmic vesicle; nucleus

Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; neurotransmitter biosynthetic process; response to corticosterone stimulus; response to pyrethroid; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; eye photoreceptor cell development; organ morphogenesis; circadian sleep/wake cycle; response to ethanol; response to zinc ion; response to activity; cerebral cortex development; response to water deprivation; response to peptide hormone stimulus; synaptic transmission, dopaminergic; locomotory behavior; fatty acid metabolic process; response to estradiol stimulus; regulation of heart contraction; response to salt stress; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; learning; response to herbicide; response to ether; memory; synaptic vesicle amine transport; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis

Disease: Segawa Syndrome, Autosomal Recessive