Mouse anti-Human, Mouse STAT 1 Monoclonal Antibody | anti-SOAT1 antibody

STAT 1, phosphorylated (Tyr701) (Signal Transducer and Activator of Transcription 1)

Cat. Num. AAA633102

• Gene Names: SOAT1; ACAT; SOAT; STAT; ACACT; ACAT1; ACAT-1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: STAT 1; Monoclonal Antibody; phosphorylated (Tyr701) (Signal Transducer and Activator of Transcription 1); Anti -STAT 1; anti-SOAT1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3G272
• Specificity: Recognizes the tyrosine-701 phosphorylated form of STAT1 protein. Does not exhibit appreciable crossreactivity with corresponding tyrosine phosphorylated forms of other STAT proteins or with other endogenous phosphotyrosine containing proteins. Species Crossreactivity: Human and mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

• Applicable Applications for anti-SOAT1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; ELISA: 0.1-1ug/ml; Immunohistochemistry (Formalin-fixed, paraffin-embedded sections): 5ug/ml; Heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required.
• Immunogen: A synthetic tyrosine phosphorylated peptide encompassing the conserved C-terminal tyrosine phosphorylation site (Y701) of murine STAT1 protein. This peptide differs from the corresponding human STAT1 sequence by one amino acid.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SOAT1 antibody

In unstimulated cells, STAT proteins exist largely in the cytoplasm as latent transcription factors. In response to treatment of target cells with cytokines or in some cases growth factors, STATs undergo tyrosine phosphorylation, homo-or heterodimerization, nuclear translocation, and DNA binding which results in transcriptional activation of distinct target genes. Phosphorylation of a conserved tyrosine residue located near the carboxy-terminus of all STAT proteins is required for both dimerization and DNA binding. Tyrosine phosphorylation is therefore a useful marker for STAT activation. At least one and oftentimes several STAT proteins are activated in response to cytokines that utilize receptors from the cytokine receptor superfamily. Nevertheless, a striking specificity of specific STAT activation is seen in response to individual cytokines. Stimulation of responsive cells with IFNa/b induces the formation of a transcription complex termed ISGF3. This complex binds to the interferon-stimulated response element (ISRE), activating the transcription of responsive genes. The ISGF3 complex consists of tyrosine phosphorylated STAT1a, STAT1b (p84), STAT2 and p48, a 48kD DNA binding protein that is specific for the IFN-stimulated response element. Formation of this complex and its migration into the nucleus is dependent upon tyrosine phosphorylation of STAT1a/b and STAT2. Stimulation of cells with IFN-g results in tyrosine phosphorylation of STAT1a (p91), but not of STAT2. Phosphorylation of STAT1a results in its homodimerization and migration into the nucleus where it binds to the IFN-g activated site (GAS). The STAT1a and STAT1b isoforms arise by alternative splicing of a single gene. The only difference between the two proteins is that STAT1b lacks 38 carboxy-terminal amino acids. Contained within these 38 terminal amino acids of STAT1a is a critical serine residue (Ser- 727) whose phosphorylation is required for maximal IFN-g induced transcription.

Product Categories/Family for anti-SOAT1 antibody

Antibodies; Abs to Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 49533617
• NCBI GeneID: 6646
• NCBI Accession #: NP_003092.4
• NCBI GenBank Nucleotide #: NM_003101.5
• UniProt Accession #: P35610; Q5T0X4; Q8N1E4; A6NC40; A8K3P4; A9Z1V7; B4DU95
• Molecular Weight: 64,735 Da
• NCBI Official Full Name: sterol O-acyltransferase 1 isoform 1
• NCBI Official Synonym Full Names: sterol O-acyltransferase 1
• NCBI Official Symbol: SOAT1
• NCBI Official Synonym Symbols: ACAT; SOAT; STAT; ACACT; ACAT1; ACAT-1
• NCBI Protein Information: sterol O-acyltransferase 1; acyl-coenzyme A:cholesterol acyltransferase 1; sterol O-acyltransferase (acyl-Coenzyme A: cholesterol acyltransferase) 1
• UniProt Protein Name: Sterol O-acyltransferase 1
• Protein Family: Sterol O-acyltransferase
• UniProt Gene Name: SOAT1
• UniProt Synonym Gene Names: ACACT; ACACT1; ACAT; ACAT1; SOAT; STAT; ACAT-1
• UniProt Entry Name: SOAT1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

SOAT1: Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Protein type: Transferase; Membrane protein, multi-pass; Membrane protein, integral; EC 2.3.1.26; Lipid Metabolism - steroid biosynthesis

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; acyl-CoA binding; sterol O-acyltransferase activity; cholesterol binding

Biological Process: cholesterol metabolic process; cholesterol homeostasis; cholesterol efflux; positive regulation of amyloid precursor protein biosynthetic process