Mouse anti-Human Prolactin, Epitope C Monoclonal Antibody
Prolactin, Epitope C (PRL)
Reactivity
Human
Applications
ELISA, Immunohistochemistry, immunoassay, Radioimmunoassay
Purity
PurifiedPurified
Synonyms
Prolactin; Epitope C; Monoclonal Antibody; Epitope C (PRL); Anti -Prolactin; anti-Prolactin; Epitope C antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
INN-hPRL-1
Specificity
Recognizes human prolactin, binding to epitope 'c'.
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-Prolactin, Epitope C antibody
ELISA (EL/EIA), Immunohistochemistry (IHC), Radioimmunoassay (RIA)
Application Notes
Suitable for use in RIA, ELISA and Immunohistochemistry.
Dilution: ELISA: 1:20-1:200
Immunohistochemistry (Frozen, paraffin): 1:500-1:1000 Requires antigen retrieval using heat treatment with 1mM EDTA, pH 8 prior to staining of paraffin sections.
Dilution: ELISA: 1:20-1:200
Immunohistochemistry (Frozen, paraffin): 1:500-1:1000 Requires antigen retrieval using heat treatment with 1mM EDTA, pH 8 prior to staining of paraffin sections.
Immunogen
Human prolactin
Positive Control
Human Pituitary
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Prolactin, Epitope C antibody
Human Prolactin (lactogenic hormone) is secreted from the anterior pituitary gland in both men and women. Human Prolactin is a single chain polypeptide hormone with a molecular weight of approximately 23,000. The release and synthesis of Prolactin is under neuroendocrinal control, primarily through Prolactin Releasing Hormone and Prolactin Inhibiting Hormone. Women normally have slightly higher basal Prolactin levels than men. Apparently, there is an estrogen-related rise at puberty and a corresponding decrease at menopause. The primary functions of Prolactin are to initiate breast development and to maintain lactation. Prolactin also suppresses gonadal function. During pregnancy, Prolactin levels increase progressively to between 10 and 20 times normal values, declining to non-pregnant levels by 3-4 weeks post-partum. Breastfeeding mothers maintain high levels of Prolactin, and it may take several months for serum concentrations to return to non-pregnant levels.
The determination of Prolactin concentration is helpful in identifying hypothalamic-pituitary disorders. Microadenomas (small pituitary tumors) may cause hyperprolactinemia, which is sometimes associated with male impotence. High Prolactin levels are commonly associated with galactorrhea and amenorrhea. Prolactin concentrations have been shown to be increased by estrogens, thyrotropin-releasing hormone (TRH), and several drugs affecting dopaminergic mechanisms. Prolactin levels are elevated in renal disease and hypothyroidism, and in some situations of stress, exercise and hypoglycemia. Additionally, the release of Prolactin is episodic and demonstrates diurnal variation. Mildly elevated Prolactin concentrations should be evaluated taking these considerations into account. Prolactin concentrations may also be increased by drugs such as chloropromazine and reserpine and may be lowered by bromocriptine and L-dopa.
The determination of Prolactin concentration is helpful in identifying hypothalamic-pituitary disorders. Microadenomas (small pituitary tumors) may cause hyperprolactinemia, which is sometimes associated with male impotence. High Prolactin levels are commonly associated with galactorrhea and amenorrhea. Prolactin concentrations have been shown to be increased by estrogens, thyrotropin-releasing hormone (TRH), and several drugs affecting dopaminergic mechanisms. Prolactin levels are elevated in renal disease and hypothyroidism, and in some situations of stress, exercise and hypoglycemia. Additionally, the release of Prolactin is episodic and demonstrates diurnal variation. Mildly elevated Prolactin concentrations should be evaluated taking these considerations into account. Prolactin concentrations may also be increased by drugs such as chloropromazine and reserpine and may be lowered by bromocriptine and L-dopa.
Product Categories/Family for anti-Prolactin, Epitope C antibody
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Product Notes
The Prolactin, Epitope C (Catalog #AAA633072) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Prolactin, Epitope C (PRL) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Prolactin, Epitope C can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC), Radioimmunoassay (RIA). Suitable for use in RIA, ELISA and Immunohistochemistry. Dilution: ELISA: 1:20-1:200 Immunohistochemistry (Frozen, paraffin): 1:500-1:1000 Requires antigen retrieval using heat treatment with 1mM EDTA, pH 8 prior to staining of paraffin sections. Researchers should empirically determine the suitability of the Prolactin, Epitope C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prolactin, Epitope C, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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