Rabbit anti-Human, Mouse P2RX7 Polyclonal Antibody | anti-P2RX7 antibody

P2RX7, CT (P2RX7, P2X purinoceptor 7, ATP receptor, P2Z receptor, Purinergic receptor) (Azide free) (HRP)

Cat. Num. AAA6330245

• Gene Names: P2RX7; P2X7
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: P2RX7; Polyclonal Antibody; CT (P2RX7; P2X purinoceptor 7; ATP receptor; P2Z receptor; Purinergic receptor) (Azide free) (HRP); Purinergic receptor; anti-P2RX7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-P2RX7 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: P2RX7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 570-600 amino acids from the C-terminal region of human P2RX7.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(P2RX7 Antibody (C-term) western blot analysis in MDA-MB435 cell line and mouse kidney tissue lysates (15ug/lane).This demonstrates the P2RX7 antibody detected the P2RX7 protein (arrow).)

Immunohistochemistry (IHC)

(P2RX7 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the P2RX7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-P2RX7 antibody

P2RX7 belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression.

Product Categories/Family for anti-P2RX7 antibody

Antibodies; Receptors

References

Kim, M., et al. EMBO J. 20(22):6347-6358(2001). Gartland, A., et al. J. Bone Miner. Res. 16(5):846-856(2001). Gu, B.J., et al. J. Biol. Chem. 276(14):11135-11142(2001). Buell, G.N., et al. Recept. Channels 5(6):347-354(1998). Rassendren, F., et al. J. Biol. Chem. 272(9):5482-5486(1997).

NCBI and Uniprot Product Information

• NCBI GI #: 29294631
• NCBI GeneID: 5027
• NCBI Accession #: NP_002553.2
• NCBI GenBank Nucleotide #: NM_002562.4
• UniProt Accession #: Q99572; O14991; Q4VKH8; Q4VKH9; Q4VKI0; Q4VKI1; Q4VKI2; A8K2Z0; E7EMK6; F5H6P2; F5H7E8; F8W951
• Molecular Weight: 58,239 Da
• NCBI Official Full Name: P2X purinoceptor 7
• NCBI Official Synonym Full Names: purinergic receptor P2X, ligand-gated ion channel, 7
• NCBI Official Symbol: P2RX7
• NCBI Official Synonym Symbols: P2X7
• NCBI Protein Information: P2X purinoceptor 7; ATP receptor; P2Z receptor; P2X7 receptor; purinergic receptor P2X7 variant A
• UniProt Protein Name: P2X purinoceptor 7
• Protein Family: P2X purinoceptor
• UniProt Gene Name: P2RX7
• UniProt Synonym Gene Names: P2X7
• UniProt Entry Name: P2RX7_HUMAN

NCBI Description

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]

Uniprot Description

P2X7: a receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis by macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Alternatively spliced variants encoding different isoforms have been identified. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. Phosphorylation results in its inactivation.

Protein type: Receptor, misc.; Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: protein complex; membrane; cell soma; integral to plasma membrane; cytoplasm; plasma membrane; terminal button; integral to nuclear inner membrane; intercellular junction; neuromuscular junction; lipid raft; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; copper ion binding; zinc ion binding; lipopolysaccharide binding; magnesium ion binding; ATP binding; purinergic nucleotide receptor activity; ATP-gated cation channel activity; receptor binding

Biological Process: activation of MAPK activity; positive regulation of T cell mediated cytotoxicity; cytolysis; positive regulation of glutamate secretion; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; sensory perception of pain; protein amino acid phosphorylation; positive regulation of interleukin-1 alpha secretion; cell surface receptor linked signal transduction; multicellular organismal protein catabolic process; ceramide biosynthetic process; T cell homeostasis; positive regulation of cytolysis; response to electrical stimulus; positive regulation of gamma-aminobutyric acid secretion; response to drug; mitochondrion organization and biogenesis; collagen metabolic process; positive regulation of interleukin-6 production; positive regulation of cytoskeleton organization and biogenesis; regulation of sodium ion transport; protein oligomerization; phagolysosome formation; phospholipid translocation; membrane depolarization; defense response to Gram-positive bacterium; NAD transport; response to mechanical stimulus; response to zinc ion; cell volume homeostasis; response to calcium ion; phospholipid transfer to membrane; cell morphogenesis; generation of action potential; regulation of killing of cells of another organism; T cell proliferation; plasma membrane organization and biogenesis; positive regulation of mitochondrial depolarization; pore complex biogenesis; inflammatory response; negative regulation of bone resorption; negative regulation of MAPKKK cascade; membrane protein ectodomain proteolysis; bleb formation; positive regulation of bone mineralization; response to ATP; synaptic vesicle exocytosis; skeletal morphogenesis; homeostasis of number of cells within a tissue; release of sequestered calcium ion into cytosol; positive regulation of prostaglandin secretion; cellular response to extracellular stimulus; innate immune response; protein processing; gene expression; membrane budding

Disease: Leukemia, Chronic Lymphocytic