Rabbit anti-Human OTUD1 Polyclonal Antibody | anti-OTUD1 antibody

OTUD1, ID (OTUD1, DUBA7, OTDC1, OTU domain-containing protein 1, DUBA-7) (Azide free) (HRP)

Cat. Num. AAA6329893

• Gene Names: OTUD1; DUBA7; OTDC1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: OTUD1; Polyclonal Antibody; ID (OTUD1; DUBA7; OTDC1; OTU domain-containing protein 1; DUBA-7) (Azide free) (HRP); DUBA-7; anti-OTUD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-OTUD1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: OTUD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 289-318 amino acids from the Central region of human OTUD1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(OTUD1 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the OTUD1 antibody detected the OTUD1 protein (arrow).)

Immunohistochemistry (IHC)

(OTUD1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OTUD1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-OTUD1 antibody

Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA7 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.[supplied by OMIM].

Product Categories/Family for anti-OTUD1 antibody

Antibodies; Proteins

References

Franke, A., et al. Gastroenterology 135(4):1207-1215(2008). Kayagaki, N., et al. Science 318(5856):1628-1632(2007). Deloukas, P., et al. Nature 429(6990):375-381(2004). Makarova, K.S., et al. Trends Biochem. Sci. 25(2):50-52(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 223941923
• NCBI GeneID: 220213
• NCBI Accession #: NP_001138845.1
• NCBI GenBank Nucleotide #: NM_001145373.2
• UniProt Accession #: Q5VV17
• Molecular Weight: 51,063 Da
• NCBI Official Full Name: OTU domain-containing protein 1
• NCBI Official Synonym Full Names: OTU deubiquitinase 1
• NCBI Official Symbol: OTUD1
• NCBI Official Synonym Symbols: DUBA7; OTDC1
• NCBI Protein Information: OTU domain-containing protein 1; DUBA-7; OTU domain containing 1
• UniProt Protein Name: OTU domain-containing protein 1
• Protein Family: OTU domain-containing protein
• UniProt Gene Name: OTUD1
• UniProt Synonym Gene Names: DUBA7; OTDC1
• UniProt Entry Name: OTUD1_HUMAN