Testing Data
Mouse RNA Polymerase II, DNA-directed, Polypeptide G Monoclonal Antibody | anti-RNA polymerase II antibody
RNA Polymerase II, DNA-directed, Polypeptide G (POLR2G, MGC138367, MGC138369, RPB7, hsRPB7, hRPB19)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Western Blot: 0.2-2ug/ml
Flow Cytometry: 0.5-2ug/sample
Testing Data
Testing Data
Western Blot (WB)
(Detection of POLR2G by Western blot. Samples: Whole cell lysate from human HT-1080 (H, 25ug) , mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells. Predicted molecular weight: 19kD)
Western Blot (WB)
(Detection of POLR2G by Western blot. Samples: Whole cell lysate from human HT-1080 (H, 25ug) , mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells. Predicted molecular weight: 19kD)
Testing Data
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-POLR2G mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG.)
Testing Data
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-POLR2G mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG.)
NCBI and Uniprot Product Information
Uniprot Description
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA
By similarity.
Subunit structure: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex
By similarity.
Subcellular location: Nucleus
By similarity.
Sequence similarities: Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.
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Product Notes
The RNA polymerase II rna polymerase ii subunit b7 (Catalog #AAA632861) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RNA Polymerase II, DNA-directed, Polypeptide G (POLR2G, MGC138367, MGC138369, RPB7, hsRPB7, hRPB19) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RNA Polymerase II, DNA-directed, Polypeptide G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS), Gel Shift Assay (GS/EMSA). Suitable for use in Western Blot, Dot Blot and Flow Cytometry. Dilution: Western Blot: 0.2-2ug/ml Flow Cytometry: 0.5-2ug/sample. Researchers should empirically determine the suitability of the RNA polymerase II rna polymerase ii subunit b7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNA Polymerase II, DNA-directed, Polypeptide G, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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