Mouse Glycogen Synthase Kinase 3 beta Monoclonal Antibody | anti-GSK3B antibody

Glycogen Synthase Kinase 3 beta, phosphorylated (Ser9) (GSK3b)

Cat. Num. AAA632817

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Purified; Purified
• Synonyms: Glycogen Synthase Kinase 3 beta; Monoclonal Antibody; phosphorylated (Ser9) (GSK3b); Anti -Glycogen Synthase Kinase 3 beta; anti-GSK3B antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2T5
• Specificity: Recognizes human phosphorylated GSK3b (pSer9) at 46kD.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, PEG, sucrose, 0.09% sodium azide, 50% glycerol.

• Applicable Applications for anti-GSK3B antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.; Dilution: Western Blot: 0.5-2ug/ml detects phosphorylated GSK3b (pSer9) in RIPA lysates from Hep G2 cells stimulated with 100uM pervandate and also from rat L6 cells stimulated with 15nM IGF-1.Non-stimulated HUVAC cell lysate and pervanadate-stimulated HUVAC cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with G8170-43 (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Immunogen: Synthetic peptide corresponding to aa6-12 (RTT[pS]FAE) of human GSK3b (KLH). The immunizing sequence is identical in mouse, rat and Xenopus.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WB: Non-stimulated L6 cell lysate (lane 1) and IGF-stimulated L6 cell lysate (lane 2) was probed with MBS632817 (2ug/ml).)

Related Product Information for anti-GSK3B antibody

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase that was first characterized by its role in intermediary metabolism. The enzyme is the mammalian homolog of the zeste-white3 (shaggy) homeotic gene of Drosophila melanogaster and has been implicated in the regulation of the c-Jun/AP-1 transcription factor. In mammals, this protein serine kinase is encoded by two related genes called GSK-3a (51kD) and b (46kD). Genetic and biochemical evidence now indicates that GSK-3 plays a role in intracellular signaling pathways. For example, during insulin and growth factor signaling the GSK-3 beta isoform becomes phosphorylated on its N-terminal serine-9 residue, presumably by either the action of MAPKAP Kinase-1 (p90 S6 kinase, Rsk-2) or p70 S6 kinase. Such phosphorylation of GSK-3 blocks its ability to phosphorylate c-Jun, protein phosphatase inhibitor 2 or the GSK-3 substrate peptide.

Product Categories/Family for anti-GSK3B antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 5730335
• NCBI GeneID: 2932
• UniProt Accession #: P49841; Q9BWH3; Q9UL47; D3DN89; Q6FI27
• Molecular Weight: 46,744 Da
• NCBI Official Full Name: glycogen synthase kinase 3 beta
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; OTTHUMP00000165239; OTTHUMP00000165240
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• Protein Family: Glycogen synthase kinase
• UniProt Gene Name: GSK3B
• UniProt Entry Name: GSK3B_HUMAN

NCBI Description

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, CMGC; EC 2.7.11.26; CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 3q13.3

Cellular Component: centrosome; dendritic spine; ribonucleoprotein complex; beta-catenin destruction complex; cytosol; lipid raft; nucleoplasm; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus

Molecular Function: p53 binding; beta-catenin binding; protein kinase binding; integrin binding; ionotropic glutamate receptor binding; protein serine/threonine kinase activity; NF-kappaB binding; protein binding; ubiquitin protein ligase binding; tau-protein kinase activity; kinase activity; tau protein binding; ATP binding

Biological Process: circadian rhythm; glycogen metabolic process; fat cell differentiation; negative regulation of MAP kinase activity; axon guidance; re-entry into mitotic cell cycle; genetic imprinting; nerve growth factor receptor signaling pathway; positive regulation of protein binding; positive regulation of axon extension; response to lithium ion; protein amino acid autophosphorylation; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; positive regulation of cell-matrix adhesion; negative regulation of protein binding; ER overload response; myoblast fusion; negative regulation of glycogen biosynthetic process; response to drug; epidermal growth factor receptor signaling pathway; regulation of microtubule-based process; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; hippocampus development; negative regulation of dendrite morphogenesis; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; organ morphogenesis; peptidyl-serine phosphorylation; negative regulation of neuron maturation; positive regulation of protein complex assembly; establishment of cell polarity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; innate immune response; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein complex assembly; negative regulation of apoptosis; regulation of neuronal synaptic plasticity