Rabbit anti-Human NUBP1 Polyclonal Antibody | anti-NUBP1 antibody

NUBP1, ID (NUBP1, NBP, NBP1, Cytosolic Fe-S cluster assembly factor NUBP1, Nucleotide-binding protein 1) (AP)

Cat. Num. AAA6327051

• Gene Names: NUBP1; NBP; NBP1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NUBP1; Polyclonal Antibody; ID (NUBP1; NBP; NBP1; Cytosolic Fe-S cluster assembly factor NUBP1; Nucleotide-binding protein 1) (AP); Nucleotide-binding protein 1; anti-NUBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-NUBP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NUBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-212 amino acids from the Central region of human NUBP1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NUBP1 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the NUBP1 antibody detected the NUBP1 protein (arrow).)

Related Product Information for anti-NUBP1 antibody

NUBP1 is a member of the NUBP/MRP subfamily of ATP-binding proteins (Nakashima et al., 1999 [PubMed 10486206]).[supplied by OMIM].

Product Categories/Family for anti-NUBP1 antibody

Antibodies; Binding Proteins

References

Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009). Shen, M., et al. Environ. Mol. Mutagen. 50(4):285-290(2009). Hosgood, H.D. III, et al. Carcinogenesis 29(10):1938-1943(2008). Stehling, O., et al. Mol. Cell. Biol. 28(17):5517-5528(2008). Taniguchi, N., et al. J. Biol. Chem. 275(41):31674-31681(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 118572611
• NCBI GeneID: 4682
• NCBI Accession #: NP_002475.2
• NCBI GenBank Nucleotide #: NM_002484.2
• UniProt Accession #: P53384; Q32M30; Q498A9; Q53FS7
• Molecular Weight: 34,534 Da
• NCBI Official Full Name: cytosolic Fe-S cluster assembly factor NUBP1
• NCBI Official Synonym Full Names: nucleotide binding protein 1
• NCBI Official Symbol: NUBP1
• NCBI Official Synonym Symbols: NBP; NBP1
• NCBI Protein Information: cytosolic Fe-S cluster assembly factor NUBP1; NBP 1; nucleotide-binding protein 1; nucleotide binding protein (e.coli MinD like); nucleotide binding protein 1 (E.coli MinD like); nucleotide binding protein 1 (MinD homolog, E. coli)
• UniProt Protein Name: Cytosolic Fe-S cluster assembly factor NUBP1
• Protein Family: Cytosolic Fe-S cluster assembly factor
• UniProt Gene Name: NUBP1
• UniProt Synonym Gene Names: NBP 1
• UniProt Entry Name: NUBP1_HUMAN

Uniprot Description

NUBP1: Implicated in the regulation of centrosome duplication. Component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. May bind and transfer 2 labile 4Fe-4S clusters to target apoproteins. Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation

Chromosomal Location of Human Ortholog: 16p13.13

Cellular Component: cytoplasm; cytosol; plasma membrane

Molecular Function: iron-sulfur cluster binding; nucleotide binding; protein binding

Biological Process: cell growth; cellular iron ion homeostasis; iron-sulfur cluster assembly