Rabbit anti-Human NR1I3 Polyclonal Antibody | anti-NR1I3 antibody

NR1I3, ID (NR1I3, CAR, Nuclear receptor subfamily 1 group I member 3, Constitutive activator of retinoid response, Constitutive androstane receptor, Orphan nuclear receptor MB67) (PE)

Cat. Num. AAA6326533

• Gene Names: NR1I3; CAR; CAR1; MB67
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NR1I3; Polyclonal Antibody; ID (NR1I3; CAR; Nuclear receptor subfamily 1 group I member 3; Constitutive activator of retinoid response; Constitutive androstane receptor; Orphan nuclear receptor MB67) (PE); Orphan nuclear receptor MB67; anti-NR1I3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NR1I3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NR1I3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the Central region of human NR1I3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NR1I3 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the NR1I3 antibody detected the NR1I3 protein (arrow).)

Related Product Information for anti-NR1I3 antibody

This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-NR1I3 antibody

Antibodies; Receptors

References

Benet, M., et al. J. Biol. Chem. 285(37):28457-28471(2010). Oliver, P., et al. Clin. Chem. Lab. Med. 48(5):635-639(2010). Masuyama, H., et al. Mol. Endocrinol. 24(4):745-753(2010). Mutoh, S., et al. J. Biol. Chem. 284(50):34785-34792(2009). Urano, T., et al. Geriatr Gerontol Int 9(3):235-241(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4826661
• NCBI GeneID: 9970
• NCBI Accession #: NP_005113.1; NP_001070948.1
• NCBI GenBank Nucleotide #: NM_005122.4
• UniProt Accession #: Q14994; Q0VAC9; Q4U0F0; E9PB75; E9PC13; E9PDU3; E9PGH6; E9PH10; E9PHC8; E9PHN4; F1D8Q0; F1D8Q1
• Molecular Weight: 30,262 Da
• NCBI Official Full Name: nuclear receptor subfamily 1 group I member 3 isoform 3
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group I, member 3
• NCBI Official Symbol: NR1I3
• NCBI Official Synonym Symbols: CAR; CAR1; MB67
• NCBI Protein Information: nuclear receptor subfamily 1 group I member 3; constitutive active receptor; constitutive active response; orphan nuclear receptor MB67; orphan nuclear hormone receptor; constitutive androstane receptor; constitutive activator of retinoid response; consti
• UniProt Protein Name: Nuclear receptor subfamily 1 group I member 3
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR1I3
• UniProt Synonym Gene Names: CAR; Constitutive active response; CAR
• UniProt Entry Name: NR1I3_HUMAN

NCBI Description

This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NR1I3: Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element. Interacts with ECT2. Heterodimer of NR1I3 and RXR. Interacts with PSMC4. Directly interacts with DNAJC7. The DNAJC7-NR1I3 complex may also include HSP90. By dexamethasone. Predominantly expressed in liver. Belongs to the nuclear hormone receptor family. NR1 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: nucleoplasm; cytoskeleton; cytoplasm; nucleus; cytosol

Molecular Function: ligand-dependent nuclear receptor activity; androgen receptor activity; DNA binding; zinc ion binding; transcription coactivator activity; thyroid hormone receptor activity; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; androgen receptor signaling pathway; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of transcription, DNA-dependent