Rabbit NR1I3 Antibody | anti-NR1I3 antibody

NR1I3 Antibody

Cat. Num. AAA2529344

• Gene Names: NR1I3; CAR; CAR1; MB67
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity purification
• Synonyms: NR1I3; NR1I3 Antibody; CAR; CAR1; MB67; anti-NR1I3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

• Applicable Applications for anti-NR1I3 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IF: 1:50 - 1:200
• Immunogen: Recombinant protein of human NR1I3
• Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NR1I3 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using NR1I3 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-NR1I3 antibody

This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 46854858
• NCBI GeneID: 9970
• Molecular Weight: Calculated MW: 30-40kDa; Observed MW: 46kDa
• NCBI Official Full Name: NR1I3 protein
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group I, member 3
• NCBI Official Symbol: NR1I3
• NCBI Official Synonym Symbols: CAR; CAR1; MB67
• NCBI Protein Information: nuclear receptor subfamily 1 group I member 3; constitutive activator of retinoid response; constitutive active receptor; constitutive active response; constitutive androstane nuclear receptor variant 2; constitutive androstane nuclear receptor variant 3; constitutive androstane nuclear receptor variant 4; constitutive androstane nuclear receptor variant 5; constitutive androstane receptor; orphan nuclear hormone receptor; orphan nuclear receptor MB67
• UniProt Protein Name: Nuclear receptor subfamily 1 group I member 3
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR1I3
• UniProt Synonym Gene Names: CAR; Constitutive active response; CAR
• UniProt Entry Name: NR1I3_HUMAN

NCBI Description

This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NR1I3: Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element. Interacts with ECT2. Heterodimer of NR1I3 and RXR. Interacts with PSMC4. Directly interacts with DNAJC7. The DNAJC7-NR1I3 complex may also include HSP90. By dexamethasone. Predominantly expressed in liver. Belongs to the nuclear hormone receptor family. NR1 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: nucleoplasm; cytoskeleton; cytoplasm; nucleus; cytosol

Molecular Function: ligand-dependent nuclear receptor activity; androgen receptor activity; DNA binding; zinc ion binding; transcription coactivator activity; thyroid hormone receptor activity; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; androgen receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; negative regulation of transcription, DNA-dependent; signal transduction