Mouse anti-Human EPHB2 Monoclonal Antibody | anti-EPHB2 antibody

EPHB2 (Ephrin Type-B Receptor 2, Tyrosine-protein Kinase Receptor EPH-3, DRT, Receptor Protein-Tyrosine Kinase HEK5, ERK, EPH-like Kinase 5, EK5, Tyrosine-protein Kinase TYRO5, Renal Carcinoma Antigen NY-REN-47, DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5)

Cat. Num. AAA632528

• Gene Names: EPHB2; DRT; EK5; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5; MGC87492
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry
• Purity: Purified by Protein G affinity chromatography followed by dialysis against PBS
• Synonyms: EPHB2; Monoclonal Antibody; EPHB2 (Ephrin Type-B Receptor 2; Tyrosine-protein Kinase Receptor EPH-3; DRT; Receptor Protein-Tyrosine Kinase HEK5; ERK; EPH-like Kinase 5; EK5; Tyrosine-protein Kinase TYRO5; Renal Carcinoma Antigen NY-REN-47; EPHT3; EPTH3; HEK5; TYRO5); Anti -EPHB2 (Ephrin Type-B Receptor 2; anti-EPHB2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 10i37
• Specificity: Recognizes human EPHB2.
• Purity/Purification: Purified by Protein G affinity chromatography followed by dialysis against PBS
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-EPHB2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC)
• Application Notes: Suitable for use in ELISA, Western Blot, Indirect Flow Cytometry, and Immunohistochemistry.; Dilution: Western Blot: 1:1000; ELISA: 1:4,000; Immunohistochemistry (FFPE): 1:200; Immunohistochemistry: 1:400; Indirect Flow Cytometry: 1:10-1:50; Other applications not tested.; Optimal dilutions to be determined by the researcher.
• Immunogen: Purified His-tagged protein mapping to a fragment of residues within amino acids 127-425 from human EPHB2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of EPHB2 recombinant protein (Fragment) using MBS632528 (1:2000).|EPHB2 (Fragment) protein (arrow) was detected using MBS632528.)

Western Blot (WB)

(Western Blot analysis of EPHB2 (arrow) using MBS632528. Lane 1: Nontransfected 293 cell lysates (2ug/lane) |Lane 2: transfected 293 cell lysates (2ug/lane) with the EphB2 (Origene Technologies).)

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin-fixed and paraffin-embedded human lung carcinoma tissue using MBS632528, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS632528 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow ytometric analysis of HepG2 cells using MBS632528 (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.)

Related Product Information for anti-EPHB2 antibody

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.

Product Categories/Family for anti-EPHB2 antibody

Antibodies; Abs to Tyrosine Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 111118978
• NCBI GeneID: 2048
• NCBI Accession #: NP_059145.2
• NCBI GenBank Nucleotide #: NM_017449.3
• UniProt Accession #: P29323; O43477; Q5T0U6; Q5T0U7; Q5T0U8; Q4LE53; Q6NVW1
• NCBI Official Full Name: ephrin type-B receptor 2 isoform 1
• NCBI Official Synonym Full Names: EPH receptor B2
• NCBI Official Symbol: EPHB2
• NCBI Official Synonym Symbols: DRT; EK5; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5; MGC87492
• NCBI Protein Information: ephrin type-B receptor 2; EPH-like kinase 5; OTTHUMP00000002914; OTTHUMP00000002915; OTTHUMP00000002916; OTTHUMP00000002917; eph tyrosine kinase 3; elk-related tyrosine kinase; protein-tyrosine kinase HEK5; tyrosine-protein kinase TYRO5; renal carcinoma antigen NY-REN-47; tyrosine-protein kinase receptor EPH-3
• UniProt Protein Name: Ephrin type-B receptor 2
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB2
• UniProt Synonym Gene Names: DRT; EPHT3; EPTH3; ERK; HEK5; TYRO5
• UniProt Entry Name: EPHB2_HUMAN

NCBI Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq]

Uniprot Description

EphB2: a receptor tyrosine kinase of the Eph family. A receptor for ephrin-B family members. Activated EphB2 recruits RasGAP, down-regulating the Ras-Erk signaling axis and neurite retraction. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB2 interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. Point mutations seen in prostate cancer. Overexpressed and required for migration of glioblastoma. Overexpressed and correlated with poor survival in breast cancer. Overexpression and loss of heterozygosity seen in colorectal cancers. Target for immunoconjugate drug therapy .Three splice-variant isoforms have been described.

Protein type: EC 2.7.10.1; Protein kinase, TK; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); Tumor suppressor; TK group; Eph family

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: cell soma; axon; integral to plasma membrane; dendrite; extracellular region; plasma membrane; cytosol

Molecular Function: protein binding; transmembrane-ephrin receptor activity; protein-tyrosine kinase activity; axon guidance receptor activity; ATP binding; receptor binding

Biological Process: axon guidance; nervous system development; inner ear morphogenesis; peptidyl-tyrosine phosphorylation; axonal fasciculation; central nervous system projection neuron axonogenesis; optic nerve morphogenesis; learning; palate development; positive regulation of long-term neuronal synaptic plasticity; corpus callosum development; positive regulation of synaptogenesis; regulation of body fluid levels; camera-type eye morphogenesis; ephrin receptor signaling pathway; negative regulation of axonogenesis; angiogenesis; phosphorylation; urogenital system development; retinal ganglion cell axon guidance

Disease: Prostate Cancer/brain Cancer Susceptibility