Rabbit anti-Human NFKBIB Polyclonal Antibody | anti-NFKBIB antibody

NFKBIB, CT (NFKBIB, IKBB, TRIP9, NF-kappa-B inhibitor beta, I-kappa-B-beta, Thyroid receptor-interacting protein 9) (Biotin)

Cat. Num. AAA6325072

• Gene Names: NFKBIB; IKBB; TRIP9
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NFKBIB; Polyclonal Antibody; CT (NFKBIB; IKBB; TRIP9; NF-kappa-B inhibitor beta; I-kappa-B-beta; Thyroid receptor-interacting protein 9) (Biotin); Thyroid receptor-interacting protein 9; anti-NFKBIB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-NFKBIB antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NFKBIB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 325-355 amino acids from the C-terminal region of human NFKBIB.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NFKBIB Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NFKBIB antibody detected the NFKBIB protein (arrow).)

Immunohistochemistry (IHC)

(NFKBIB Antibody (C-term) (AP13780b)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NFKBIB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-NFKBIB antibody

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

Product Categories/Family for anti-NFKBIB antibody

Antibodies; Protein Kinase Inhibitors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Potter, C., et al. Ann. Rheum. Dis. 69(7):1315-1320(2010). Segat, L., et al. Vaccine 28(10):2201-2206(2010). McGeachie, M., et al. Circulation 120(24):2448-2454(2009). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 48928017
• NCBI GeneID: 4793
• UniProt Accession #: Q15653; Q59EM7; Q96LS1
• Molecular Weight: 32,803 Da
• NCBI Official Full Name: NF-kappa-B inhibitor beta isoform b [Homo sapiens]
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
• NCBI Official Symbol: NFKBIB
• NCBI Official Synonym Symbols: IKBB; TRIP9
• NCBI Protein Information: NF-kappa-B inhibitor beta; ikB-B; TRIP-9; ikB-beta; ikappaBbeta; NF-kappa-BIB; I-kappa-B-beta; TR-interacting protein 9; thyroid receptor-interacting protein 9
• UniProt Protein Name: NF-kappa-B inhibitor beta
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBIB
• UniProt Synonym Gene Names: IKBB; TRIP9; NF-kappa-BIB; IkB-B; IkB-beta; IkappaBbeta; TR-interacting protein 9; TRIP-9
• UniProt Entry Name: IKBB_HUMAN

Uniprot Description

IkB-beta: a protein of the NF-kappa-B inhibitor family. Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA- dependent inactivation. Association with inhibitor kappa B- interacting Ras-like proteins 1 and 2 may also explain the slower degradation. Interacts with ligand-binding domain of thyroid hormone receptor-beta, inhibitor kappa B-interacting Ras-like protein 1, inhibitor kappa B-interacting protein Ras2, p65 (RELA) and c-Rel. Expressed in all tissues examined.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: nucleus; cytosol

Molecular Function: signal transducer activity; protein binding; transcription coactivator activity

Biological Process: transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor signaling pathway; positive regulation of interferon type I production; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway