Rabbit anti-Human NFIL3 Polyclonal Antibody | anti-NFIL3 antibody

NFIL3, NT (NFIL3, E4BP4, IL3BP1, Nuclear factor interleukin-3-regulated protein, E4 promoter-binding protein 4, Interleukin-3 promoter transcriptional activator, Interleukin-3-binding protein 1, Transcriptional activator NF-IL3A) (APC)

Cat. Num. AAA6325027

• Gene Names: NFIL3; E4BP4; IL3BP1; NFIL3A; NF-IL3A
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NFIL3; Polyclonal Antibody; NT (NFIL3; E4BP4; IL3BP1; Nuclear factor interleukin-3-regulated protein; E4 promoter-binding protein 4; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; Transcriptional activator NF-IL3A) (APC); Transcriptional activator NF-IL3A; anti-NFIL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-NFIL3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NFIL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-74 amino acids from the N-terminal region of human NFIL3.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NFIL3 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NFIL3 antibody detected the NFIL3 protein (arrow).)

Related Product Information for anti-NFIL3 antibody

Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).

Product Categories/Family for anti-NFIL3 antibody

Antibodies; Transcription Factors

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Weng, Y.J., et al. Mol. Cell. Biochem. 340 (1-2), 187-194 (2010): Lavebratt, C., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (2), 570-581 (2010): Utge, S.J., et al. PLoS ONE 5 (2), E9259 (2010): Mansour, H.A., et al. Bipolar Disord 11(7):701-710(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 52630429
• NCBI GeneID: 4783
• NCBI Accession #: NP_005375.2
• NCBI GenBank Nucleotide #: NM_005384.2
• UniProt Accession #: Q16649; Q14211; Q6FGQ8; Q96HS0; B2R9Y8
• Molecular Weight: 51,472 Da
• NCBI Official Full Name: nuclear factor interleukin-3-regulated protein
• NCBI Official Synonym Full Names: nuclear factor, interleukin 3 regulated
• NCBI Official Symbol: NFIL3
• NCBI Official Synonym Symbols: E4BP4; IL3BP1; NFIL3A; NF-IL3A
• NCBI Protein Information: nuclear factor interleukin-3-regulated protein; E4 promoter-binding protein 4; interleukin-3-binding protein 1; transcriptional activator NF-IL3A; nuclear factor interleukin 3 regulated protein; interleukin-3 promoter transcriptional activator
• UniProt Protein Name: Nuclear factor interleukin-3-regulated protein
• Protein Family: Nuclear factor interleukin-3-regulated protein
• UniProt Gene Name: NFIL3
• UniProt Synonym Gene Names: E4BP4; IL3BP1
• UniProt Entry Name: NFIL3_HUMAN

NCBI Description

Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).[supplied by OMIM, Feb 2009]

Uniprot Description

E4BP4: Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B- site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death. Belongs to the bZIP family. NFIL3 subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: nucleus

Molecular Function: DNA binding; transcription corepressor activity; transcription factor activity

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; immune response; negative regulation of transcription from RNA polymerase II promoter