Mouse anti-Human, Rat Heat Shock Protein 90, alpha Monoclonal Antibody | anti-HSP90AB1 antibody

Heat Shock Protein 90, alpha (HSP90a)

Cat. Num. AAA632498

• Gene Names: HSP90AB1; HSP90
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Heat Shock Protein 90; alpha; Monoclonal Antibody; alpha (HSP90a); Anti -Heat Shock Protein 90; anti-HSP90AB1 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 7L17
• Specificity: Recognizes human HSP90 alpha at ~90kD. Does not crossreact with HSP90 beta. Species Crossreactivity: rat
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

• Applicable Applications for anti-HSP90AB1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry.; Dilution: Western Blot: 1ug/ml for detection in 20ug of HeLa lysate.
• Immunogen: Recombinant human HSP90 alpha
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-HSP90AB1 antibody

HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms alpha and beta, which share 85% sequence amino acid homology. The two isoforms of Hsp90 are expressed in the cytosolic compartment. Despite the similarities, HSP90 exists predominantly as a homodimer while HSP90b exists mainly as a monomer. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. Furthermore, Hsp90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1-2% of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90- regulated proteins that have been discovered to date are involved in cell signaling. The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function.

Product Categories/Family for anti-HSP90AB1 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 126352614
• NCBI GeneID: 100034150
• NCBI Accession #: NP_001075407.1
• NCBI GenBank Nucleotide #: NM_001081938.1
• UniProt Accession #: Q9GKX8; Q6TXV3
• Molecular Weight: 83,237 Da
• NCBI Official Full Name: heat shock protein HSP 90-beta
• NCBI Official Symbol: HSP90AB1
• NCBI Official Synonym Symbols: HSP90
• NCBI Protein Information: heat shock protein HSP 90-beta; heat shock protein 90; Heat shock protein HSP 90-beta-like protein
• UniProt Protein Name: Heat shock protein HSP 90-beta
• Protein Family: Heat shock protein
• UniProt Gene Name: HSP90AB1
• UniProt Synonym Gene Names: HSPCB
• UniProt Entry Name: HS90B_HORSE

Uniprot Description

Function: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function

By similarity.

Subunit structure: Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4

By similarity.

Subcellular location: Cytoplasm

By similarity. Melanosome

By similarity.

Domain: The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins

By similarity.

Post-translational modification: ISGylated

By similarity.Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro)

By similarity.S-nitrosylated; negatively regulates the ATPase activity

By similarity.

Sequence similarities: Belongs to the heat shock protein 90 family.