Rabbit anti-Human NDFIP1 Polyclonal Antibody | anti-NDFIP1 antibody

NDFIP1, ID (NDFIP1, N4WBP5, NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M, NEDD4 WW domain-binding protein 5, Putative MAPK-activating protein PM13, Putative NF-kappa-B-activating protein 164, Putative NFKB and MAPK-activatin

Cat. Num. AAA6324238

• Gene Names: NDFIP1; N4WBP5
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NDFIP1; Polyclonal Antibody; ID (NDFIP1; N4WBP5; NEDD4 family-interacting protein 1; Breast cancer-associated protein SGA-1M; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NF-kappa-B-activating protein 164; Putative NFKB and MAPK-activatin; Putative NFKB and MAPK-activating protein; anti-NDFIP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-NDFIP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NDFIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-106 amino acids from the Central region of human NDFIP1.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-NDFIP1 antibody

The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins.

Product Categories/Family for anti-NDFIP1 antibody

Antibodies; Cancer Markers

References

Mund, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(25):11429-11434(2010). Howitt, J., et al. Proc. Natl. Acad. Sci. U.S.A. 106(36):15489-15494(2009). Rapley, E.A., et al. Nat. Genet. 41(7):807-810(2009). Mund, T., et al. EMBO Rep. 10(5):501-507(2009). Putz, U., et al. J. Biol. Chem. 283(47):32621-32627(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 13386480
• NCBI GeneID: 80762
• NCBI Accession #: NP_085048.1
• NCBI GenBank Nucleotide #: NM_030571.3
• UniProt Accession #: Q9BT67; Q658T8; Q8N2E3; Q8N2F9; B2RDB8; D3DQF0
• Molecular Weight: 24,899 Da
• NCBI Official Full Name: NEDD4 family-interacting protein 1
• NCBI Official Synonym Full Names: Nedd4 family interacting protein 1
• NCBI Official Symbol: NDFIP1
• NCBI Official Synonym Symbols: N4WBP5
• NCBI Protein Information: NEDD4 family-interacting protein 1; Nedd4 WW domain-binding protein 5; putative MAPK-activating protein PM13; breast cancer-associated protein SGA-1M; putative NFKB and MAPK-activating protein; putative NF-kappa-B-activating protein 164
• UniProt Protein Name: NEDD4 family-interacting protein 1
• Protein Family: NEDD4 family-interacting protein
• UniProt Gene Name: NDFIP1
• UniProt Synonym Gene Names: N4WBP5
• UniProt Entry Name: NFIP1_HUMAN

NCBI Description

The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

NDFIP1: Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation. In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: Golgi membrane; perinuclear region of cytoplasm; extracellular region; integral to membrane; endosome membrane; cell cortex

Molecular Function: signal transducer activity; protein binding; WW domain binding

Biological Process: negative regulation of interleukin-4 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; cellular iron ion homeostasis; regulation of lymphocyte differentiation; regulation of myeloid leukocyte differentiation; signal transduction; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell proliferation; negative regulation of T-helper 2 type immune response; positive regulation of protein ubiquitination; negative regulation of inflammatory response; positive regulation of protein catabolic process; negative regulation of protein transport; regulation of isotype switching to IgG isotypes; negative regulation of transporter activity