Rabbit anti-Human MGMT Polyclonal Antibody | anti-MGMT antibody

MGMT, NT (MGMT, Methylated-DNA-protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase) (PE)

Cat. Num. AAA6320689

• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MGMT; Polyclonal Antibody; NT (MGMT; Methylated-DNA-protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase) (PE); Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA-alkyltransferase; anti-MGMT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MGMT antibody: Immunofluorescence (IF), FLISA, Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MGMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MGMT.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MGMT Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MGMT Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-MGMT antibody

Antibodies; Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 127069
• NCBI GeneID: 4255
• UniProt Accession #: P16455; Q5VY78
• Molecular Weight: 21,646 Da
• NCBI Official Full Name: Methylated-DNA--protein-cysteine methyltransferase
• NCBI Official Synonym Full Names: O-6-methylguanine-DNA methyltransferase
• NCBI Official Symbol: MGMT
• NCBI Protein Information: methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase
• UniProt Protein Name: Methylated-DNA--protein-cysteine methyltransferase
• Protein Family: Probable methylated-DNA--protein-cysteine methyltransferase
• UniProt Gene Name: MGMT
• UniProt Synonym Gene Names: MGMT
• UniProt Entry Name: MGMT_HUMAN

Uniprot Description

MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.

Protein type: EC 2.1.1.63; Methyltransferase; Nuclear receptor co-regulator; DNA repair, damage; Apoptosis

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: methyltransferase activity; DNA binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity

Biological Process: response to drug; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to folic acid; response to toxin; DNA methylation; regulation of caspase activity; DNA repair; DNA ligation