Rabbit anti-Human MEKK4 Polyclonal Antibody | anti-MEKK4 antibody

MEKK4, ID (C1081) (MAP3K4, KIAA0213, MAPKKK4, MEKK4, MTK1, Mitogen-activated protein kinase kinase kinase 4, MAP three kinase 1, MAPK/ERK kinase kinase 4) (Azide free) (HRP)

Cat. Num. AAA6320203

• Gene Names: MAP3K4; MTK1; MEKK4; MEKK 4; MAPKKK4; PRO0412
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MEKK4; Polyclonal Antibody; ID (C1081) (MAP3K4; KIAA0213; MAPKKK4; MTK1; Mitogen-activated protein kinase kinase kinase 4; MAP three kinase 1; MAPK/ERK kinase kinase 4) (Azide free) (HRP); MAP3K4; MAPK/ERK kinase kinase 4; anti-MEKK4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 1558

• Applicable Applications for anti-MEKK4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MEKK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1065-1097 amino acids from the Central region of human MEKK4.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MEKK4 Antibody MBS6035191 western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the MEKK4 antibody detected the MEKK4 protein (arrow).)

Immunohistochemistry (IHC)

(MEKK4 Antibody (Center C1081) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MEKK4 Antibody (Center C1081) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-MEKK4 antibody

Antibodies; Protein Kinases

References

Grucza, R.A., et al. Addict Biol 15(3):346-357(2010). Whitmarsh, A.J., et al. Oncogene 26(22):3172-3184(2007). Aissouni, Y., et al. Biochem. Biophys. Res. Commun. 338(2):808-814(2005). Abell, A.N., et al. J. Biol. Chem. 280(43):35793-35796(2005). Derbyshire, Z.E., et al. Mol. Cell. Biochem. 271 (1-2), 77-90 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 55956902
• NCBI GeneID: 4216
• NCBI Accession #: NP_006715.2
• NCBI GenBank Nucleotide #: NP_006715.2
• UniProt Accession #: Q9Y6R4
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 4 isoform b
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 4
• NCBI Official Symbol: MAP3K4
• NCBI Official Synonym Symbols: MTK1; MEKK4; MEKK 4; MAPKKK4; PRO0412
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 4
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 4
• UniProt Gene Name: MAP3K4
• UniProt Synonym Gene Names: KIAA0213; MAPKKK4; MEKK4; MTK1; MEK kinase 4; MEKK 4
• UniProt Entry Name: M3K4_HUMAN

NCBI Description

The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, May 2014]

Uniprot Description

MEKK4: a protein kinase of the STE11 family. A mediator of environmental stresses. Phosphorylates and activates MKK4, -6, and-7, which in turn activate the p38 AND JNK MAPK pathways.

Protein type: Protein kinase, STE; EC 2.7.11.25; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); STE group; STE11 family

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: perinuclear region of cytoplasm; cytoplasm

Molecular Function: MAP kinase kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; ATP binding

Biological Process: positive regulation of JNK activity; activation of MAPKK activity; activation of MAPK activity; regulation of gene expression; MAPKKK cascade; protein amino acid phosphorylation; response to UV-C; placenta development; male germ-line sex determination