Rabbit anti-Human MEKK4 Polyclonal Antibody | anti-MAP3K4 antibody

MEKK4, ID (C1081) (MAP3K4, KIAA0213, MAPKKK4, MEKK4, MTK1, Mitogen-activated protein kinase kinase kinase 4, MAP three kinase 1, MAPK/ERK kinase kinase 4)

Cat. Num. AAA641600

• Gene Names: MAP3K4; MTK1; MEKK4; MAPKKK4; PRO0412
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: MEKK4; Polyclonal Antibody; ID (C1081) (MAP3K4; KIAA0213; MAPKKK4; MTK1; Mitogen-activated protein kinase kinase kinase 4; MAP three kinase 1; MAPK/ERK kinase kinase 4); Anti -MEKK4; anti-MAP3K4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-MAP3K4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: MEKK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1065-1097 amino acids from the Central region of human MEKK4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MEKK4 Antibody (C1081) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the MEKK4 antibody detected the MEKK4 protein (arrow).)

Immunohistochemistry (IHC)

(MEKK4 Antibody (Center C1081) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MEKK4 Antibody (Center C1081) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-MAP3K4 antibody

The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described.

Product Categories/Family for anti-MAP3K4 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 55956904
• NCBI GeneID: 4216
• NCBI Accession #: NP_005913.2
• NCBI GenBank Nucleotide #: NM_005922.2
• UniProt Accession #: Q9Y6R4; Q5VTT8; Q5VTT9; Q92612; Q9H408; A6H8W0; B7ZLD3; B9EG75
• Molecular Weight: 181,685 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 4 isoform a
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 4
• NCBI Official Symbol: MAP3K4
• NCBI Official Synonym Symbols: MTK1; MEKK4; MAPKKK4; PRO0412
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 4; MEKK 4; MEK kinase 4; MAP three kinase 1; MAP/ERK kinase kinase 4; MAPK/ERK kinase kinase 4; predicted protein of HQ0412; SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of; dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4)
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 4
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K4
• UniProt Synonym Gene Names: KIAA0213; MAPKKK4; MEKK4; MTK1; MEK kinase 4; MEKK 4
• UniProt Entry Name: M3K4_HUMAN

NCBI Description

The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

MEKK4: a protein kinase of the STE11 family. A mediator of environmental stresses. Phosphorylates and activates MKK4, -6, and-7, which in turn activate the p38 AND JNK MAPK pathways.

Protein type: Protein kinase, STE; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; STE group; STE11 family

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: perinuclear region of cytoplasm; cytoplasm

Molecular Function: MAP kinase kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; ATP binding

Biological Process: positive regulation of JNK activity; activation of MAPKK activity; activation of MAPK activity; regulation of gene expression; MAPKKK cascade; protein amino acid phosphorylation; response to UV-C; male germ-line sex determination; placenta development