Rabbit anti-Human MCF2 Polyclonal Antibody | anti-MCF2 antibody

MCF2, CT (MCF2, DBL, Proto-oncogene DBL, Proto-oncogene MCF-2, MCF2-transforming protein, DBL-transforming protein) (MaxLight 405)

Cat. Num. AAA6319611

• Gene Names: MCF2; DBL; ARHGEF21
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MCF2; Polyclonal Antibody; CT (MCF2; DBL; Proto-oncogene DBL; Proto-oncogene MCF-2; MCF2-transforming protein; DBL-transforming protein) (MaxLight 405); DBL-transforming protein; anti-MCF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MCF2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
• Sequence Length: 860

• Applicable Applications for anti-MCF2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 804-833 in the C-terminal region of human MCF2, Swiss/UniProt Accession: P10911
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6008662. This demonstrates that MBS6008662 detected the MCF2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6008662 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6008662 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of K562 cells using MBS6008662 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-MCF2 antibody

Antibodies; Proteins

References

1. Piton, A., et al. Mol. Psychiatry (2010). In press: 2. Murakami, M., et al. Int. J. Cancer 123(3):500-510(2008). 3. Murakami, M., et al. Cancer Biol. Ther. 7(5):677-688(2008). 4. Rojas, R.J., et al. J. Biol. Chem. 282(40):29201-29210(2007). 5. Prag, S., et al. Mol. Biol. Cell 18(8):2935-2948(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 284795243
• NCBI GeneID: 4168
• NCBI Accession #: NP_001165349.1; NP_005360.3
• NCBI GenBank Nucleotide #: NP_001165349.1
• UniProt Accession #: P10911
• NCBI Official Full Name: proto-oncogene DBL isoform e
• NCBI Official Synonym Full Names: MCF.2 cell line derived transforming sequence
• NCBI Official Symbol: MCF2
• NCBI Official Synonym Symbols: DBL; ARHGEF21
• NCBI Protein Information: proto-oncogene DBL
• UniProt Protein Name: Proto-oncogene DBL
• Protein Family: Proto-oncogene
• UniProt Gene Name: MCF2
• UniProt Synonym Gene Names: DBL
• UniProt Entry Name: MCF2_HUMAN

NCBI Description

The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.[provided by RefSeq, Jan 2010]